Difference between revisions of "Part:BBa K3740008"
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<partinfo>BBa_K3740008 short</partinfo>
 
<partinfo>BBa_K3740008 short</partinfo>
 
===Description===
 
===Description===
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Modulation of protein expression level
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Modulation of protein expression level.
  
 
===Sequence and Features===
 
===Sequence and Features===
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=2021 SZPT-China=
 
=2021 SZPT-China=
 
<h3>Biology</h3>
 
<h3>Biology</h3>
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<p>Mutagenesis at specific position of RBS (<partinfo>BBa_B0034</partinfo>) was achieved RBS009 (<partinfo>BBa_K3740008</partinfo>) using random primers and PCR, and the constructed plasmid J23100-RBS009-sfGFP-rrn B T1 (<partinfo>BBa_K3740060</partinfo>）. Compare the fluorescence intensity of sfGFP induced by J23100-B0034-sfGFP-rrn B T1 (<partinfo>BBa_K3740058</partinfo>)</p>
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<p>Mutagenesis at specific position of RBS (<partinfo>BBa_B0034</partinfo>) was achieved RBS009 (<partinfo>BBa_K3740008</partinfo>) using random primers and PCR, and the constructed plasmid J23100-RBS009-sfGFP-rrnB T1 (<partinfo>BBa_K3740060</partinfo>). Compare the fluorescence intensity of sfGFP induced by J23100-B0034-sfGFP-rrnB T1 (<partinfo>BBa_K3740058</partinfo>).</p>
 
<br>
 
<br>
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<h3>Usage
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<h3>Usage</h3>
 +
Constructed J23100-RBS009-sfGFP-rrnB T1 (<partinfo>BBa_K3740060</partinfo>) was transformed into <i>E. coli</i> DH5α. We quantified the
 +
fluorescence intensity when the optical absorbance of bacterial culture at 600nm was around 0.2.
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<br>
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<h3>Characterization</h3>
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<p>The average fluorescence intensity of sfGFP induced by RBS009 (<partinfo>BBa_K3740008</partinfo>), was less than 3% of B0034 (<partinfo>BBa_B0034</partinfo>),<b> which means reducing the pressure to the host bacteria</b>.</p>
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[[File:szpt0.png|300px|thumb|center|Significance analysis of the average fluorescence intensity of sfGFP between B0034 and RBS009]]
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<h3>References</h3>
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<p>[1] Zhang H M ,  Chen S ,  Shi H , et al. Measurements of Gene Expression at Steady State Improve the Predictability of Part Assembly[J]. Acs Synthetic Biology, 2016, 5(3):269.</p>

Latest revision as of 11:04, 21 October 2021


RBS009

Description

Modulation of protein expression level.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


2021 SZPT-China

Biology

Mutagenesis at specific position of RBS (BBa_B0034) was achieved RBS009 (BBa_K3740008) using random primers and PCR, and the constructed plasmid J23100-RBS009-sfGFP-rrnB T1 (BBa_K3740060). Compare the fluorescence intensity of sfGFP induced by J23100-B0034-sfGFP-rrnB T1 (BBa_K3740058).


Usage

Constructed J23100-RBS009-sfGFP-rrnB T1 (BBa_K3740060) was transformed into E. coli DH5α. We quantified the fluorescence intensity when the optical absorbance of bacterial culture at 600nm was around 0.2.

Characterization

The average fluorescence intensity of sfGFP induced by RBS009 (BBa_K3740008), was less than 3% of B0034 (BBa_B0034), which means reducing the pressure to the host bacteria.

Significance analysis of the average fluorescence intensity of sfGFP between B0034 and RBS009

References

[1] Zhang H M ,  Chen S ,  Shi H , et al. Measurements of Gene Expression at Steady State Improve the Predictability of Part Assembly[J]. Acs Synthetic Biology, 2016, 5(3):269.