Difference between revisions of "Part:BBa K3889102"

Latest revision as of 14:13, 19 October 2021

Ovastacin expression cassette Bacillus subtilis

P43 (BBa_K143013) fused with P22 binding site L (BBa_K3889091) and RBS (BBa_K1351030) in order to create P22 c2 repressed Ovastacin expression. Also in order to secrete Ovastacin PhoD signal peptide (BBa_K3889050) was also fused.

Usage and Biology

This part along with BBa_K3889100 and BBa_K3889101 was used in order to produce Ovastacin (BBa_K3889022) when progesterone in absent in the fallopian tube. The working of the system is shown below:

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3889102 short</partinfo>
-P22 regulated Ovastacin expression and fluorescence check cassette in Bacillus subtilis.
+P43 (<partinfo>BBa_K143013</partinfo>) fused with P22 binding site L (<partinfo>BBa_K3889091</partinfo>) and RBS (<partinfo>BBa_K1351030</partinfo>) in order to create P22 c2 repressed Ovastacin expression. Also in order to secrete Ovastacin PhoD signal peptide (<partinfo>BBa_K3889050</partinfo>) was also fused.
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
-<!-- -->
+This part along with <partinfo>BBa_K3889100</partinfo> and <partinfo>BBa_K3889101</partinfo> was used in order to produce Ovastacin (<partinfo>BBa_K3889022</partinfo>) when progesterone in absent in the fallopian tube. The working of the system is shown below:
+<html>
+<body>
+<video style="width: 100%" muted id="myVideo" controls autoplay loop>
+<source src="https://static.igem.org/mediawiki/parts/c/c7/T--IISER-Tirupati_India--ovastacin_production.mov">
+</video>
+</html>
 <span class='h3bb'>Sequence and Features</span>
 <partinfo>BBa_K3889102 SequenceAndFeatures</partinfo>