Difference between revisions of "Part:BBa K3930006"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K3930006 short</partinfo> | <partinfo>BBa_K3930006 short</partinfo> | ||
+ | <span class='h3bb'>Sequence and Features</span> | ||
+ | <partinfo>BBa_K3930006 SequenceAndFeatures</partinfo> | ||
− | + | <html> | |
− | This sequence must be used | + | <h2>Introduction</h2> |
− | + | <p>The Z3eV assembly is composed of the promoter TEF1, the gene <i>Z3eV</i> coding for the activator Z3eV of the estradiol inducible promoter BBa_K3930007 and the transcription terminator of CYC1. The construction is based on the publication (Liu et al., 2020). </p> | |
+ | <p>This sequence must be used with the promoter Z3eV <a href="https://parts.igem.org/Part:BBa_K3930007" class="pr-0" target="_blank">(BBa_K3930007)</a>, for a gene regulated expression into <i>S. cerevisiae</i>. | ||
+ | <br> | ||
+ | <h2>Testing</h2> | ||
+ | <p>This part is one of the component of the pFLEUR part <a href="https://parts.igem.org/Part:BBa_K3930000" class="pr-0" target="_blank">(BBa_K3930000)</a> and has been succesfully cloned into the LycoYeast <i>S. cerevisiae</i> strain genome. The Toulouse team could not test this part for reasons of time, and preferred to focus on the part characterizations that allow the production of α and β-ionone for the floral aspect of the violet perfume.</p> | ||
+ | <br> | ||
+ | <h2>References</h2> | ||
+ | <ol> | ||
+ | <i> | ||
+ | <li>Liu J, Wang T, Jiang Y, Liu Z, Tian P, Wang F, Deng L. 2020. Harnessing β-estradiol inducible expression system to overproduce nervonic acid in Saccharomyces cerevisiae. Process Biochemistry. 92:37–42. doi:10.1016/j.procbio.2020.02.032. | ||
+ | </li> | ||
+ | </i> | ||
+ | </ol> | ||
+ | </html> | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
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Latest revision as of 17:04, 16 October 2021
Inducer Z3EV of the estradiol promoter
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 1021
Illegal XbaI site found at 1324
Illegal PstI site found at 666
Illegal PstI site found at 837
Illegal PstI site found at 1340 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 666
Illegal PstI site found at 837
Illegal PstI site found at 1340 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 1021
Illegal XbaI site found at 1324
Illegal PstI site found at 666
Illegal PstI site found at 837
Illegal PstI site found at 1340 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 1021
Illegal XbaI site found at 1324
Illegal PstI site found at 666
Illegal PstI site found at 837
Illegal PstI site found at 1340
Illegal AgeI site found at 1527 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1849
Illegal BsaI.rc site found at 987
Introduction
The Z3eV assembly is composed of the promoter TEF1, the gene Z3eV coding for the activator Z3eV of the estradiol inducible promoter BBa_K3930007 and the transcription terminator of CYC1. The construction is based on the publication (Liu et al., 2020).
This sequence must be used with the promoter Z3eV (BBa_K3930007), for a gene regulated expression into S. cerevisiae.
Testing
This part is one of the component of the pFLEUR part (BBa_K3930000) and has been succesfully cloned into the LycoYeast S. cerevisiae strain genome. The Toulouse team could not test this part for reasons of time, and preferred to focus on the part characterizations that allow the production of α and β-ionone for the floral aspect of the violet perfume.
References
- Liu J, Wang T, Jiang Y, Liu Z, Tian P, Wang F, Deng L. 2020. Harnessing β-estradiol inducible expression system to overproduce nervonic acid in Saccharomyces cerevisiae. Process Biochemistry. 92:37–42. doi:10.1016/j.procbio.2020.02.032.