Difference between revisions of "Part:BBa K3889010"

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<partinfo>BBa_K3889010 short</partinfo>
 
<partinfo>BBa_K3889010 short</partinfo>
  
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This is a synthetically derived promoter sequence for the expression of genes in <i>Bacillus subtilis</i>.
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This is a synthetically derived promoter sequence for constitutive expression of genes in <i>Bacillus subtilis</i>.
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{| class=wikitable style="text-align: center;"
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|-
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!Promoter
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!Sequence 5‘ → 3‘
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!Relative activity wrt P43 of GFP (%)
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!Standard deviation
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|-
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|SP126
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|AAAAATTATAAAAATGTGTTGACAAAGGGGGTCCTGTATGTTATAATAGCTT
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|29.07
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|0.23
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|-
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|SP146
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|AAAAATAACAAAAACGTGTTGACAATAAAGATTAACCGTGATATAATTAAAT
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|40.39
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|0.69
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|-
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|SP200
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|AAAAATTAGAAAAATGTGTTGACACTCGGACGAAACAATGGTATAATGGCAA
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|76.82
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|0.9
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|}
  
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[[Media:T--IISER-Tirupati_India--Synthetic_promoter_of_Bacillus_subtilis.pdf]]
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[[Media:T--IISER-Tirupati_India--Synthetic_promoter_of_Bacillus_subtilis.pdf|Synthetic Promoters data from SP1-214]][1]
  
  
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<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
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SP126(<partinfo>BBa_K3889010</partinfo>), SP146(<partinfo>BBa_K3889011</partinfo>) and SP200(<partinfo>BBa_K3889012</partinfo>) are a part of synthetic library promoters from Liu et al. (2018)[1]. These synthetic promoters are characterised to have constitutive expression, hence they can be used in general protein production. The library contains 214 synthetic promoters providing variability and robustness in expression, according to the system's need. All SP promoters have the following consensus sequences:
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div.scroll {overflow-x: aut0; overflow-y: hidden;}
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<img src="https://static.igem.org/mediawiki/parts/8/88/T--IISER-Tirupati_India--SP_backbone.jpeg">
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Based on this the promoters are as follows:
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[[File:T--IISER-Tirupati India--SP allignment.png]]<br>
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Fig source: Geneious version 2021.2 created by Biomatters. Available from https://www.geneious.com
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===Modelling===
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We planned to use SP126 with <partinfo>BBa_K1351033</partinfo> for gene expression of SRTF1 (<partinfo>BBa_K3889100</partinfo>).
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[[File:T--IISER-Tirupati India--SP126.jpg|900 px]]
  
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K3889010 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3889010 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K3889010 parameters</partinfo>
 
<partinfo>BBa_K3889010 parameters</partinfo>
 
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===References===
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1. Liu, D., Mao, Z., Guo, J., Wei, L., Ma, H., Tang, Y., Chen, T., Wang, Z., & Zhao, X. (2018). Construction, Model-Based Analysis, and Characterization of a Promoter Library for Fine-Tuned Gene Expression in Bacillus subtilis. ACS Synthetic Biology, 7(7), 1785–1797. https://doi.org/10.1021/acssynbio.8b00115

Latest revision as of 20:15, 18 October 2021


Bacillus Subtilis Synthetic Promoter 126

This is a synthetically derived promoter sequence for constitutive expression of genes in Bacillus subtilis.

Promoter Sequence 5‘ → 3‘ Relative activity wrt P43 of GFP (%) Standard deviation
SP126 AAAAATTATAAAAATGTGTTGACAAAGGGGGTCCTGTATGTTATAATAGCTT 29.07 0.23
SP146 AAAAATAACAAAAACGTGTTGACAATAAAGATTAACCGTGATATAATTAAAT 40.39 0.69
SP200 AAAAATTAGAAAAATGTGTTGACACTCGGACGAAACAATGGTATAATGGCAA 76.82 0.9

Synthetic Promoters data from SP1-214[1]


Usage and Biology

SP126(BBa_K3889010), SP146(BBa_K3889011) and SP200(BBa_K3889012) are a part of synthetic library promoters from Liu et al. (2018)[1]. These synthetic promoters are characterised to have constitutive expression, hence they can be used in general protein production. The library contains 214 synthetic promoters providing variability and robustness in expression, according to the system's need. All SP promoters have the following consensus sequences:


Based on this the promoters are as follows: T--IISER-Tirupati India--SP allignment.png
Fig source: Geneious version 2021.2 created by Biomatters. Available from https://www.geneious.com

Modelling

We planned to use SP126 with BBa_K1351033 for gene expression of SRTF1 (BBa_K3889100).


T--IISER-Tirupati India--SP126.jpg

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

1. Liu, D., Mao, Z., Guo, J., Wei, L., Ma, H., Tang, Y., Chen, T., Wang, Z., & Zhao, X. (2018). Construction, Model-Based Analysis, and Characterization of a Promoter Library for Fine-Tuned Gene Expression in Bacillus subtilis. ACS Synthetic Biology, 7(7), 1785–1797. https://doi.org/10.1021/acssynbio.8b00115