Difference between revisions of "Part:BBa K4047002"
 
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This is the forward primer to amplify the gene for fructose-1,6-bisphosphatase class 1 (Synpcc7942_2335). The overlap sequence (red) allows proper Gibson assembly with the pGEM-fbp backbone. The annealing portion (all caps) allows annealing to the WT S. elongatus PCC 7942 genome for proper amplification.
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__NOTOC__
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<partinfo>BBa_K4047002 short</partinfo>
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<!-- Add more about the biology of this part here
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K4047002 SequenceAndFeatures</partinfo>
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<!-- Uncomment this to enable Functional Parameter display
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===Functional Parameters===
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<partinfo>BBa_K4047002 parameters</partinfo>
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===Long Description===
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This is the forward primer paired with the reverse primer BBa_K4047003 to amplify the gene for fructose-1,6-bisphosphatase class 1 (BBa_K4047035). The overlap sequence allows proper Gibson assembly with the pGEM-fbp backbone (BBa_K4047024) to create the full plasmid pGEM-fbp (BBa_K4047037). The annealing portion (all caps) allows annealing to the wildtype Synechococcus elongatus PCC 7942 genome for proper amplification.

Latest revision as of 04:29, 16 October 2021


primerF_fbp-foward


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 20
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 20
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 20
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 20
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 20
  • 1000
    COMPATIBLE WITH RFC[1000]


Long Description

This is the forward primer paired with the reverse primer BBa_K4047003 to amplify the gene for fructose-1,6-bisphosphatase class 1 (BBa_K4047035). The overlap sequence allows proper Gibson assembly with the pGEM-fbp backbone (BBa_K4047024) to create the full plasmid pGEM-fbp (BBa_K4047037). The annealing portion (all caps) allows annealing to the wildtype Synechococcus elongatus PCC 7942 genome for proper amplification.