Difference between revisions of "Part:BBa K3804008:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | + | We used tctagaGAAAGAGGGGACAAactag in BBa_J61100 as RBS and deleted 6bp(taataa) which locate in the end of LasI. Thus, the length of this sequence is 792. | |
Latest revision as of 05:49, 30 September 2021
LasI
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 270
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We used tctagaGAAAGAGGGGACAAactag in BBa_J61100 as RBS and deleted 6bp(taataa) which locate in the end of LasI. Thus, the length of this sequence is 792.
Source
The part consists of sigma70promoter(BBa_J23100), RBS(BBa_J61100), LasI(BBa_C0178) and double terminator(BBa_B0015)