Difference between revisions of "Part:BBa K136006"
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It is activated by ''FlhD4C2'' and maybe by ''FliA'' with different strength ( Using a Quantitative Blueprint to Reprogram the Dynamics of the Flagella Gene Network - Shiraz Kalir and Uri Alon ). | It is activated by ''FlhD4C2'' and maybe by ''FliA'' with different strength ( Using a Quantitative Blueprint to Reprogram the Dynamics of the Flagella Gene Network - Shiraz Kalir and Uri Alon ). | ||
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| + | '''This part works. Check experiment done with it on the [[Part:BBa_K136006:Experience|experience]] page''' | ||
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'''WARNING'''<br> | '''WARNING'''<br> | ||
| − | The promoter cloned here | + | The promoter cloned here corresponds to the promoter region given in the following paper:<br> |
Kalir, S. et al. Ordering genes in a flagella pathway by analysis of expression kinetics from living bacteria. Science 292, 2080-2083 (2001) | Kalir, S. et al. Ordering genes in a flagella pathway by analysis of expression kinetics from living bacteria. Science 292, 2080-2083 (2001) | ||
| − | Position 1130245-1130044 in MG1655 genome sequence | + | Position 1130245-1130044 in MG1655 genome sequence. |
Note that the promoter region given here includes the beginning of the flgA protein. It thus cannot be used with any protein coding biobrick since it will add a small peptide and most probably produce a frame shift in your protein. To use it in front of an ORF, this ORF must be preceded by a stop codon in the right frame. | Note that the promoter region given here includes the beginning of the flgA protein. It thus cannot be used with any protein coding biobrick since it will add a small peptide and most probably produce a frame shift in your protein. To use it in front of an ORF, this ORF must be preceded by a stop codon in the right frame. | ||
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
Latest revision as of 04:43, 8 November 2008
flgA promoter followed by its natural RBS
flgA is a class 2 gene that is part of the construction of the flagella ( Ordering Genes in a Flagella Pathway by Analysis of Expression Kinetics from Living Bacteria - S. Kalir, J. McClure, K. Pabbaraju, C. Southward, M. Ronen, S. Leibler, M. G. Surette, U. Alon ).
Its promoter is consitutively repressed.
It is activated by FlhD4C2 and maybe by FliA with different strength ( Using a Quantitative Blueprint to Reprogram the Dynamics of the Flagella Gene Network - Shiraz Kalir and Uri Alon ).
This part works. Check experiment done with it on the experience page
WARNING
The promoter cloned here corresponds to the promoter region given in the following paper:
Kalir, S. et al. Ordering genes in a flagella pathway by analysis of expression kinetics from living bacteria. Science 292, 2080-2083 (2001)
Position 1130245-1130044 in MG1655 genome sequence.
Note that the promoter region given here includes the beginning of the flgA protein. It thus cannot be used with any protein coding biobrick since it will add a small peptide and most probably produce a frame shift in your protein. To use it in front of an ORF, this ORF must be preceded by a stop codon in the right frame.
We are currently building a new version of this promoter which will include stop codons so that it will comply with the biobrick standard and be usable in front of any protein coding biobrick.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]