Difference between revisions of "Part:BBa K3425054"

Latest revision as of 00:16, 28 October 2020

Constitutive expression construct of Juniper GFP

Asembled using Type IIS iGEM Loop assembly standard from the constitutive promoter, BBa_J23100, the RBS for Type IIS iGEM Standard Assembly, BBa_K3425031, the Juniper GFP for Type IIS iGEM standard assembly, BBa_K3425048, as well as the double terminator BBa_B0015.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 476
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 476
Illegal NheI site found at 7
Illegal NheI site found at 30
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 476
Illegal BamHI site found at 685
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 476
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 476
1000
COMPATIBLE WITH RFC[1000]

@@ Line 4: / Line 4: @@
 <html>
-Asembled using <a href="https://parts.igem.org/Help:Assembly/Scars">Type IIS iGEM Loop assembly standard</a>from the constitutive promoter, BBa_J23100, the RBS for Type IIS iGEM Standard Assembly, BBa_K3425031, the Juniper GFP for Type IIS iGEM standard assembly, BBa_K3425048, followed by the double terminator BBa_B0015.
+Asembled using <a href="https://parts.igem.org/Help:Assembly/Scars">Type IIS iGEM Loop assembly standard</a> from the constitutive promoter, BBa_J23100, the RBS for Type IIS iGEM Standard Assembly, BBa_K3425031, the Juniper GFP for Type IIS iGEM standard assembly, BBa_K3425048, as well as the double terminator BBa_B0015.
 </html>
@@ Line 13: / Line 13: @@
 <span class='h3bb'>Sequence and Features</span>
 <partinfo>BBa_K3425054 SequenceAndFeatures</partinfo>
 <!-- Uncomment this to enable Functional Parameter display