Difference between revisions of "Part:BBa K3505012"
(→Design Notes) |
|||
Line 19: | Line 19: | ||
The sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is cloned in pUPD2 <bbpart>BBa_K3505007</bbpart> and has overhangs compatible for GoldenBraid cloning. | The sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is cloned in pUPD2 <bbpart>BBa_K3505007</bbpart> and has overhangs compatible for GoldenBraid cloning. | ||
This has position A1-B3. | This has position A1-B3. | ||
− | [[File:T--Thessaly--GB-GGAG-AATG.jpeg|700px|thumb|none|<i><b>Fig. | + | [[File:T--Thessaly--GB-GGAG-AATG.jpeg|700px|thumb|none|<i><b>Fig.1:</b>The overhangs of this part in the GoldenBraid Grammar</i>]] |
− | [[File:T--Thessaly--AndersonRBS-snap.png|700px|thumb|none|<i><b>Fig. | + | [[File:T--Thessaly--AndersonRBS-snap.png|700px|thumb|none|<i><b>Fig.2:</b>J23115 with RBS</i>]] |
===Verification of cloning=== | ===Verification of cloning=== | ||
− | [[File:T--Thessaly--Anderson--digestion.png|700px|thumb|none|<i><b>Fig. | + | [[File:T--Thessaly--Anderson--digestion.png|700px|thumb|none|<i><b>Fig.3:</b>((U=Uncut , C= Cut) Restriction digestion of AndersonJ23115 with : |
EcoRI (C1a & C2a),Expected bands : 2156 bp, | EcoRI (C1a & C2a),Expected bands : 2156 bp, | ||
XhoI (C1b & C2b),Expected bands : 1264 + 892 bp | XhoI (C1b & C2b),Expected bands : 1264 + 892 bp |
Latest revision as of 00:39, 28 October 2020
AndersonJ23115:RBS GB compatible with A1-B2
Constitutive Anderson BBa_J23115with RBS BBa_B0034
AndersonJ23115 is a strong constitutive promoter that can be used to tune the expression level of constitutively expressed parts.
Design Notes
The sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is cloned in pUPD2 BBa_K3505007 and has overhangs compatible for GoldenBraid cloning. This has position A1-B3.
Verification of cloning
Experimental Use and Experience
This part is used in BBa_K3505033 BBa_K3505035
Source
Synthesized by IDT.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 11
Illegal NheI site found at 34 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]