Difference between revisions of "Part:BBa K3505012"
 
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===Design Notes===
 
===Design Notes===
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The sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is cloned in pUPD2 <bbpart>BBa_K3505007</bbpart> and has overhangs compatible for Golden Braid cloning.  
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The sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is cloned in pUPD2 <bbpart>BBa_K3505007</bbpart> and has overhangs compatible for GoldenBraid cloning.  
 
This has position A1-B3.
 
This has position A1-B3.
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[[File:T--Thessaly--GB-GGAG-AATG.jpeg|700px|thumb|none|<i><b>Fig.2:</b>The overhangs of this part in the Golden Braid Grammar</i>]]
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[[File:T--Thessaly--GB-GGAG-AATG.jpeg|700px|thumb|none|<i><b>Fig.1:</b>The overhangs of this part in the GoldenBraid Grammar</i>]]
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[[File:T--Thessaly--AndersonRBS-snap.png|700px|thumb|none|<i><b>Fig.1:</b>J23115 with RBS</i>]]
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[[File:T--Thessaly--AndersonRBS-snap.png|700px|thumb|none|<i><b>Fig.2:</b>J23115 with RBS</i>]]
  
 
===Verification of cloning===
 
===Verification of cloning===
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[[File:T--Thessaly--Anderson--digestion.png|700px|thumb|none|<i><b>Fig.1:</b>((U=Uncut , C= Cut) Restriction digestion of AndersonJ23115 with :  
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[[File:T--Thessaly--Anderson--digestion.png|700px|thumb|none|<i><b>Fig.3:</b>((U=Uncut , C= Cut) Restriction digestion of AndersonJ23115 with :  
 
EcoRI (C1a & C2a),Expected bands : 2156 bp,
 
EcoRI (C1a & C2a),Expected bands : 2156 bp,
 
XhoI (C1b & C2b),Expected bands : 1264 + 892 bp
 
XhoI (C1b & C2b),Expected bands : 1264 + 892 bp

Latest revision as of 00:39, 28 October 2020


AndersonJ23115:RBS GB compatible with A1-B2


Constitutive Anderson BBa_J23115with RBS BBa_B0034

AndersonJ23115 is a strong constitutive promoter that can be used to tune the expression level of constitutively expressed parts.

Design Notes

The sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is cloned in pUPD2 BBa_K3505007 and has overhangs compatible for GoldenBraid cloning. This has position A1-B3.

Fig.1:The overhangs of this part in the GoldenBraid Grammar
Fig.2:J23115 with RBS

Verification of cloning

Fig.3:((U=Uncut , C= Cut) Restriction digestion of AndersonJ23115 with : EcoRI (C1a & C2a),Expected bands : 2156 bp, XhoI (C1b & C2b),Expected bands : 1264 + 892 bp Positive result: The set of C1a and C1b (same sample)

Experimental Use and Experience

This part is used in BBa_K3505033 BBa_K3505035

Source

Synthesized by IDT.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 11
    Illegal NheI site found at 34
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]