Difference between revisions of "Part:BBa K3411040"

Latest revision as of 02:45, 28 October 2020

NarL Gene

Lambert_GA iGEM 2020's NarL system is modeled after E.coli’s natural nitrate sensor, the Nar Operon, which includes the NarL response regulator, and NarX, the membrane-bound sensor protein. The NarL/NarX system detects nitrate. This specific part is the isolated NarL gene.

Description

The listed NarL’s role within Lambert_GA iGEM 2020’s (improved from HKUST-Rice 2015, BBa_K1682018) system serves as an acceptor of the phosphate group from NarX. The promoter BBa_J23106, a constitutive Anderson Promoter, initiates the production of TetR. TetR then represses the PtetO promoter. By regulating the amount of aTc, the molecule that inhibits TetR, the team can then control levels of NarL. In the presence of nitrate, NarX phosphorylates NarL. Then, phosphorylated NarL binds to a specified promoter and correlates to measured nitrate levels. Further details about the continued expression of the NarL part are listed in the description HKUST-Rice provides on their part. Therefore, phosphorylated NarL measures nitrate levels.

Figure 1: Diagram of the nitrate detection NarL system construct.

Lambert_GA iGEM 2020 will continue cloning and begin characterization of the NarL part in the coming year as a part of the two-year project, AgroSense.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 174
Illegal BglII site found at 342
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3411040 short</partinfo>
-Lambert iGEM's NarL system is modeled after <i>E.coli’s</i> natural nitrite and nitrate sensor: the Nar Operon, which includes the NarL response regulator, the gene listed above, and NarX, the membrane-bound sensor protein. The NarL/NarX system detects both nitrite and nitrate. This specific part is the isolated NarL gene.
+Lambert_GA iGEM 2020's NarL system is modeled after <i>E.coli’s</i> natural nitrate sensor, the Nar Operon, which includes the NarL response regulator, and NarX, the membrane-bound sensor protein. The NarL/NarX system detects nitrate. This specific part is the isolated NarL gene.
-The listed NarL’s role within Lambert iGEM’s improved NarL System is based on HKUST-Rice 2015,
+<strong>Description</strong>
-[https://parts.igem.org/Part:BBa_K1682018 BBa_K1682018]. The promoter [https://parts.igem.org/Part:BBa_J23106 BBa_J23106],
- a constitutive Anderson Promoter, which initiates the production of TetR. TetR represses the PtetO promoter, producing NarP. In the presence of both nitrate and nitrite, NarQ phosphorylates NarP. Therefore, phosphorylated NarP measures both nitrite and nitrate levels. By using the corrected difference, our NarP and NarL (Lambert iGEM’s improved part of HKUST-Rice 2015 [https://parts.igem.org/Part:BBa_K1682018 BBa_K1682018] composite parts are able to evaluate the nitrite levels.
-Lambert iGEM plans on cloning and characterizing our NarL part in the coming year as a part of our two-year project, AgroSense.
+The listed NarL’s role within Lambert_GA iGEM 2020’s (improved from HKUST-Rice 2015, [https://parts.igem.org/Part:BBa_K1682018 BBa_K1682018]) system serves as an acceptor of the phosphate group from NarX. The promoter [https://parts.igem.org/Part:BBa_J23106 BBa_J23106], a constitutive Anderson Promoter, initiates the production of TetR. TetR then represses the PtetO promoter. By regulating the amount of aTc, the molecule that inhibits TetR, the team can then control levels of NarL. In the presence of nitrate, NarX phosphorylates NarL. Then, phosphorylated NarL binds to a specified promoter and correlates to measured nitrate levels. Further details about the continued expression of the NarL part are listed in the description HKUST-Rice provides on their part. Therefore, phosphorylated NarL measures nitrate levels.
+[[Image:NARLDESIGN.png|500px|thumb|center|<i>Figure 1: Diagram of the nitrate detection NarL system construct.</i>]]
+Lambert_GA iGEM 2020 will continue cloning and begin characterization of the NarL part in the coming year as a part of the two-year project, AgroSense.