Difference between revisions of "Part:BBa K3589107"
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+ | __NOTOC__ | ||
+ | <partinfo>BBa_K3589107 short</partinfo> | ||
+ | <html> | ||
+ | <p> | ||
+ | This basic part contains the coding sequence of the laccase from the ascomycete <I>Botrytis aclada</i> mutant L499F (B3). This part is codon-optimized for <I>Chlamydomonas reinhardtii</I>. Combined with the second part of the laccase (<a href="https://parts.igem.org/Part:BBa_K3589110">BBa_K3589110</a>), a promoter and a terminator, this basic part should mediate the oxidation of a wide variety of substrates including phenolic compounds and aromatic amines. As this part contains the introns 1 and 2 of RBCS2, it perfectly matches the part <a href="https://parts.igem.org/Part:BBa_K3002027">BBa_K3002027</a> (pAR promoter A1-B2), resulting in a high expression (<a href="https://www.researchgate.net/publication/23762345_Strategies_to_facilitate_transgene_expression_in_Chlamydomonas_reinhardtii">Eichler-Stahlberg et al., 2009</a>). | ||
+ | To detect the target protein a 3xHA-tag from the Kaiser Collection (<a href="https://parts.igem.org/Part:BBa_K3002017">BBa_K3002017</a>) is recommended. | ||
+ | We recommend using the pAR promoter (A1-A3) (<a href="https://parts.igem.org/Part:BBa_K3002003">BBa_K3002003</a>). | ||
+ | <br> | ||
+ | This part was designed to allow synthesis due to a high GC-level. It is only functional in combination with the second part (<a href="https://parts.igem.org/Part:BBa_K3589110">BBa_K3589110</a>), of the laccase. Visit the page of the part <a href="https://parts.igem.org/Part:BBa_K3589108">BBa_K3589108</a> to gain a deeper understanding of the functionality of the laccase</b> </br> | ||
+ | <p> | ||
+ | </html> | ||
+ | <!-- Add more about the biology of this part here | ||
+ | ===Usage and Biology=== | ||
+ | |||
+ | <!-- --> | ||
+ | <span class='h3bb'>Sequence and Features</span> | ||
+ | <partinfo>BBa_K3589107 SequenceAndFeatures</partinfo> | ||
+ | |||
+ | |||
+ | <!-- Uncomment this to enable Functional Parameter display | ||
+ | ===Functional Parameters=== | ||
+ | <partinfo>BBa_K3589107 parameters</partinfo> | ||
+ | <!-- --> |
Latest revision as of 13:35, 25 October 2020
Mutant laccase from Botrytis aclada for Chlamydomonas reinhardtii, Part 1 (Phytobrick)
This basic part contains the coding sequence of the laccase from the ascomycete Botrytis aclada mutant L499F (B3). This part is codon-optimized for Chlamydomonas reinhardtii. Combined with the second part of the laccase (BBa_K3589110), a promoter and a terminator, this basic part should mediate the oxidation of a wide variety of substrates including phenolic compounds and aromatic amines. As this part contains the introns 1 and 2 of RBCS2, it perfectly matches the part BBa_K3002027 (pAR promoter A1-B2), resulting in a high expression (Eichler-Stahlberg et al., 2009).
To detect the target protein a 3xHA-tag from the Kaiser Collection (BBa_K3002017) is recommended.
We recommend using the pAR promoter (A1-A3) (BBa_K3002003).
This part was designed to allow synthesis due to a high GC-level. It is only functional in combination with the second part (BBa_K3589110), of the laccase. Visit the page of the part BBa_K3589108 to gain a deeper understanding of the functionality of the laccase
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 608
Illegal PstI site found at 695
Illegal PstI site found at 759
Illegal PstI site found at 1131
Illegal PstI site found at 1419 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 608
Illegal PstI site found at 695
Illegal PstI site found at 759
Illegal PstI site found at 1131
Illegal PstI site found at 1419 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1055
Illegal BamHI site found at 1394 - 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 608
Illegal PstI site found at 695
Illegal PstI site found at 759
Illegal PstI site found at 1131
Illegal PstI site found at 1419 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 608
Illegal PstI site found at 695
Illegal PstI site found at 759
Illegal PstI site found at 1131
Illegal PstI site found at 1419 - 1000COMPATIBLE WITH RFC[1000]