Difference between revisions of "Part:BBa K3657032"

Latest revision as of 02:21, 28 October 2020

MS2 Coat Protein coupled with GFP

This is a fusion protein containing MS2 Coat Protein (BBa_K3218006) and GFP (BBa_E0040).

Usage and Biology

MS2 Coat Protein binds specifically the MS2 RNA hairpin (BBa_K3113006).

GFP is a green fluorescent protein

This construct was planned to be used as the positive controle in an experiment aiming to explore the ability to use RNA as a linker between proteins. You can find out more about the planned experiment here: iGEM Heidelberg 2020.

Due to the COVID19 pandemics we did not obtain empirical data about this part.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1049

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 <partinfo>BBa_K3657032 short</partinfo>
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+<html>
+<p>
+This is a fusion protein containing MS2 Coat Protein (<a href="https://parts.igem.org/Part:BBa_K3218006">BBa_K3218006</a>) and GFP (<a href="https://parts.igem.org/Part:BBa_E0040">BBa_E0040</a>).
+</p>
+</html>
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+<html>
+<p>MS2 Coat Protein binds specifically the MS2 RNA hairpin (<a href="https://parts.igem.org/Part:BBa_K3113006">BBa_K3113006</a>).</p>
+<p>GFP is a green fluorescent protein</p>
+<br>
+<p>This construct was planned to be used as the positive controle in an experiment aiming to explore the ability to use RNA as a linker between proteins. You can find out more about the planned experiment here: <a href="https://2020.igem.org/Team:Heidelberg/RNA_Protein">iGEM Heidelberg 2020.</a></p>
+<p> Due to the COVID19 pandemics we did not obtain empirical data about this part.</p>
-<!-- -->
+</html>
 <span class='h3bb'>Sequence and Features</span>
 <partinfo>BBa_K3657032 SequenceAndFeatures</partinfo>