Difference between revisions of "Part:BBa K3610017:Design"
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===Design Notes=== | ===Design Notes=== | ||
Codons with a frequency lower than 10% were considered rare and were replaced for optimal expression. As alternative codons, the ones with the highest frequencies were chosen unless it added unwanted restriction sites and was therefore incompatible with iGEM standards. | Codons with a frequency lower than 10% were considered rare and were replaced for optimal expression. As alternative codons, the ones with the highest frequencies were chosen unless it added unwanted restriction sites and was therefore incompatible with iGEM standards. | ||
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===Source=== | ===Source=== | ||
| − | The sequence was taken of [[Part: | + | The sequence was taken of [[Part:BBa_K1761006]] from the iGEM registry and then codon optimized with SnapGene. |
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===References=== | ===References=== | ||
Latest revision as of 03:25, 28 October 2020
SmallBit NanoLuc - codon optimized for C. reinhardtii
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Codons with a frequency lower than 10% were considered rare and were replaced for optimal expression. As alternative codons, the ones with the highest frequencies were chosen unless it added unwanted restriction sites and was therefore incompatible with iGEM standards.
Source
The sequence was taken of Part:BBa_K1761006 from the iGEM registry and then codon optimized with SnapGene.
References
England, Christopher G.; Ehlerding, Emily B.; Cai, Weibo (2016): NanoLuc: A Small Luciferase Is Brightening Up the Field of Bioluminescence. In: Bioconjug Chem 27 (5), S. 1175–1187. DOI: 10.1021/acs.bioconjchem.6b00112.
Naya, Hugo; Romero, Héctor; Carels, Nicola; Zavala, Alejandro; Musto, Héctor (2001): Translational selection shapes codon usage in the GC-rich genome of Chlamydomonas reinhardtii. In: FEBS Letters 501 (2), S. 127–130. DOI: 10.1016/S0014-5793(01)02644-8.