Difference between revisions of "Part:BBa K3117046:Design"
 
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===Design Notes===
 
===Design Notes===
 
[[File:G4S_4_Linker.gif|thumb|right|200px|'''Figure 1''': Modelling of the 4x[GGGS] linker]]
 
[[File:G4S_4_Linker.gif|thumb|right|200px|'''Figure 1''': Modelling of the 4x[GGGS] linker]]
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The part consists of humanized anti-CD3 and anti-GPA33 single-chain variable fragments (scFv) (<partinfo>BBa_K3117033</partinfo>, <partinfo>BBa_K3117035</partinfo>, <partinfo>BBa_K3117034</partinfo>, <partinfo>BBa_K3117036</partinfo>), which are connected by a flexible [GGGGS]4 linker (BBa_K3117004). The scFvs are formed by the variable regions of the heavy and light chain of the respective antibodies that are connected by either a [GGGGS]4 or [GGGGS]3 linker (<partinfo>BBa_K3117004</partinfo>, <partinfo>BBa_K3117028</partinfo>). The sequence contains a C-terminal His-Tag (BBa_K3117005) for easy purification and detection. Secretion of the protein into the periplasm is ensured by a pelB sequence (<partinfo>BBa_K3117012</partinfo>).
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The part consists of humanized anti-CD3 and anti-GPA33 single-chain variable fragments (scFv) (<partinfo>BBa_K3117020</partinfo>, <partinfo>BBa_K3117021</partinfo>, <partinfo>BBa_K3117022</partinfo>, <partinfo>BBa_K3117023</partinfo>), which are connected by a flexible [GGGGS]4 linker (BBa_K3117004). The scFvs are formed by the variable regions of the heavy and light chain of the respective antibodies that are connected by either a [GGGGS]4 or [GGGGS]3 linker (<partinfo>BBa_K3117004</partinfo>, <partinfo>BBa_K3117028</partinfo>). The sequence contains a C-terminal His-Tag (BBa_K3117005) for easy purification and detection. Secretion of the protein is ensured by an Igk leader (<partinfo>BBa_K3117006</partinfo>). When the protein passes the membrane, this leader domain is cleaved off.
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Latest revision as of 21:35, 21 October 2019


scFv bispecific antibody against GPA33 and CD3 codon optimized for E.coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 54
    Illegal AgeI site found at 519
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Figure 1: Modelling of the 4x[GGGS] linker

The part consists of humanized anti-CD3 and anti-GPA33 single-chain variable fragments (scFv) (BBa_K3117033, BBa_K3117035, BBa_K3117034, BBa_K3117036), which are connected by a flexible [GGGGS]4 linker (BBa_K3117004). The scFvs are formed by the variable regions of the heavy and light chain of the respective antibodies that are connected by either a [GGGGS]4 or [GGGGS]3 linker (BBa_K3117004, BBa_K3117028). The sequence contains a C-terminal His-Tag (BBa_K3117005) for easy purification and detection. Secretion of the protein into the periplasm is ensured by a pelB sequence (BBa_K3117012).