Coding

Part:BBa_K3117035

Designed by: Lena Schorr   Group: iGEM19_FAU_Erlangen   (2019-10-18)


scFv VL sequence of anti-CD3 antibody codon optimized for expression in E.coli

The single chain variable fragment of the heavy chain of anti-GPA33 targets the colon cancer surface marker GPA33. Glycoprotein A33 (GPA33) is a well-known surface marker of colon cancer. Under healthy conditions GPA33 is just expressed on gastric cells. On primary and metastatic colorectal cancers, GPA33 is overexpressed in 95% of primary and colorectal cancers [1].


Usage and Biology

The single chain fragment targeting GPA33 was used to be fused to the other components of the bispecific antibody to target CD3 and GPA33.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None