Difference between revisions of "Part:BBa K3137000"
 
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<partinfo>BBa_K3137000 short</partinfo>
 
<partinfo>BBa_K3137000 short</partinfo>
  
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PrsmH can detect the intensity of the fluorescence of <i>gfp</i>([https://parts.igem.org/Part:BBa_K3137011# BBa_K3137011]).
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we ligated promoter <i>rsmH</i> with <i>gfp</i>([https://parts.igem.org/Part:BBa_K3137011# BBa_K3137011]) to measure the fluorescence intensity of GFP.  
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In the case of determining that the combination resistant protein is effective, we chose the constitutive promoter PrsmH to constitutively express the resistant protein in an attempt to verify whether the resistant protein has an effect on cell growth.
 
In the case of determining that the combination resistant protein is effective, we chose the constitutive promoter PrsmH to constitutively express the resistant protein in an attempt to verify whether the resistant protein has an effect on cell growth.
  
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First, we linked this constitutive promoter PrsmH to <i>gfp</i>([https://parts.igem.org/Part:BBa_K3137011# BBa_K3137011]), demonstrating the validity of this constitutive promoter. The effect of overexpression of the resistant protein AbpAB([https://parts.igem.org/Part:BBa_K3137006# BBa_K3137006]) on growth was then verified. The results showed that PrsmH had a good effect, and overexpression of AbpAB had no significant effect on the growth of <i>E. coli</i> BL21.
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First, we linked this constitutive promoter PrsmH to <i>gfp</i>([https://parts.igem.org/Part:BBa_K3137011# BBa_K3137011]), then demonstrated the validity of this constitutive promoter. The effect of overexpression of the resistant protein AbpAB([https://parts.igem.org/Part:BBa_K3137006# BBa_K3137006]) on growth was then verified. The results showed that PrsmH had a good effect, and overexpression of AbpAB had no significant effect on the growth of <i>E. coli</i> BL21.
  
  
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[[Image:RsmH.jpeg|400px|thumb|center|Green fluorescent of <i>E. coli</i> BL21-pET28a-PrsmH-<i>gfp</i>]]
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[[Image:RsmH.jpeg|500px|thumb|center|Green fluorescent of <i>E. coli</i> BL21-pET28a-PrsmH-<i>gfp</i>]]
  
 
[[Image:AbpAB 1.png|500px|center|thumb|The effect of protein AbpAB on growth of <I>E. coli</i> BL21]]
 
[[Image:AbpAB 1.png|500px|center|thumb|The effect of protein AbpAB on growth of <I>E. coli</i> BL21]]

Latest revision as of 21:13, 21 October 2019


PrsmH

we ligated promoter rsmH with gfp(BBa_K3137011) to measure the fluorescence intensity of GFP.


Usage and Biology

In the case of determining that the combination resistant protein is effective, we chose the constitutive promoter PrsmH to constitutively express the resistant protein in an attempt to verify whether the resistant protein has an effect on cell growth.

First, we linked this constitutive promoter PrsmH to gfp(BBa_K3137011), then demonstrated the validity of this constitutive promoter. The effect of overexpression of the resistant protein AbpAB(BBa_K3137006) on growth was then verified. The results showed that PrsmH had a good effect, and overexpression of AbpAB had no significant effect on the growth of E. coli BL21.


Green fluorescent of E. coli BL21-pET28a-PrsmH-gfp
The effect of protein AbpAB on growth of E. coli BL21


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]