Difference between revisions of "Part:BBa K3286204"
 
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<partinfo>BBa_K3286204 short</partinfo>
 
<partinfo>BBa_K3286204 short</partinfo>
  
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The function of this composite part is that's a Two component system able to sense a diffused signalling factor (DSF)[1]. When DSF is present, the RpfCch will autophosphorylate and transfer the phosphate group to the enzyme RpfG. RpfG is a phosphodiesterase which is able to cleave c-di-GMP. This results in activation of this enzyme, which will decrease the amount of c-di-GMP in the cell.
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The function of this composite part is that's a Two component system able to sense a diffused signalling factor (DSF)[1]. The sensor is called RpfC(ch) (<partinfo>BBa_K3286200</partinfo>).When DSF is present, the RpfCch will autophosphorylate and transfer the phosphate group to the enzyme RpfG. RpfG (<partinfo>BBa_K3286201</partinfo>) is a phosphodiesterase which is able to cleave c-di-GMP. This results in activation of this enzyme, which will decrease the amount of c-di-GMP in the cell.
  
  

Latest revision as of 23:45, 20 October 2019


Two component system RpfCch + RpfG

The function of this composite part is that's a Two component system able to sense a diffused signalling factor (DSF)[1]. The sensor is called RpfC(ch) (BBa_K3286200).When DSF is present, the RpfCch will autophosphorylate and transfer the phosphate group to the enzyme RpfG. RpfG (BBa_K3286201) is a phosphodiesterase which is able to cleave c-di-GMP. This results in activation of this enzyme, which will decrease the amount of c-di-GMP in the cell.


  1. H. Slater, A. Alvarez-Morales, C. E. Barber, M. J. Daniels, and J. Maxwell Dow, “A two-component system involving an HD-GYP domain protein links cell-cell signalling to pathogenicity gene expression in Xanthomonas campestris,” Mol. Microbiol., vol. 38, no. 5, pp. 986–1003, 2000.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1922
    Illegal PstI site found at 887
    Illegal PstI site found at 1493
    Illegal PstI site found at 2120
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1922
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal PstI site found at 887
    Illegal PstI site found at 1493
    Illegal PstI site found at 2120
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1922
    Illegal BglII site found at 288
    Illegal BglII site found at 3362
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1922
    Illegal PstI site found at 887
    Illegal PstI site found at 1493
    Illegal PstI site found at 2120
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1922
    Illegal PstI site found at 887
    Illegal PstI site found at 1493
    Illegal PstI site found at 2120
    Illegal NgoMIV site found at 818
    Illegal NgoMIV site found at 909
    Illegal NgoMIV site found at 1736
    Illegal NgoMIV site found at 1831
    Illegal NgoMIV site found at 2890
  • 1000
    COMPATIBLE WITH RFC[1000]