Coding

Part:BBa_K3286201

Designed by: Ben Kuipers   Group: iGEM19_Wageningen_UR   (2019-10-19)


RpfG

This is the coding sequence of RpfG. RpfG is part of a two-component system, it can be activated by RpfC(ch)(BBa_K3286200, BBa_K1315002). RpfG is a phosphodiesterase which is able to cleave c-di-GMP, when activated. There is already a part present coding for an RpfG in the repository under BBa_K1315003.


  1. M. O. Andrade et al., “The HD-GYP domain of RpfG mediates a direct linkage between the Rpf quorum-sensing pathway and a subset of diguanylate cyclase proteins in the phytopathogen Xanthomonas axonopodis pv citri,” Mol. Microbiol., vol. 62, no. 2, pp. 537–551, 2006.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1091
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 619
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None