Difference between revisions of "Part:BBa K3187044"
 
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This part is a generator for the P22 bacteriophage coat protein fused to a LPETGG-tag for Sortase-mediated ligation of GGGG-tagged proteins. This part was used together with <a
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This part is a generator for the P22 bacteriophage coat protein fused to a LPETGG-tag for sortase-mediated ligation of GGGG-tagged proteins. This part was used together with <a
 
                     href=" https://parts.igem.org/Part:BBa_K3187043" target="_blank">BBa_K3187043</a> for the dual expression of the P22 scaffold protein and the P22 coat protein to generated P22 Virus-like particles, that can be modified on their surface using sortase-mediated ligation. The potential of fine tuning the expression levels of both parts was tested via <a
 
                     href=" https://parts.igem.org/Part:BBa_K3187043" target="_blank">BBa_K3187043</a> for the dual expression of the P22 scaffold protein and the P22 coat protein to generated P22 Virus-like particles, that can be modified on their surface using sortase-mediated ligation. The potential of fine tuning the expression levels of both parts was tested via <a
 
                     href=" https://parts.igem.org/Part:BBa_K3187011" target="_blank">BBa_K3187011</a>.
 
                     href=" https://parts.igem.org/Part:BBa_K3187011" target="_blank">BBa_K3187011</a>.
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<span class='h3bb'>Sequence and Features</span>
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<h2><span class='h3bb'>Sequence and Features</span></h2>
 
<partinfo>BBa_K3187044 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3187044 SequenceAndFeatures</partinfo>
  

Latest revision as of 08:54, 20 October 2019


P22 Bacteriophag Coat Protein (T7 promotor)

This part is a generator for the P22 bacteriophage coat protein fused to a LPETGG-tag for sortase-mediated ligation of GGGG-tagged proteins. This part was used together with BBa_K3187043 for the dual expression of the P22 scaffold protein and the P22 coat protein to generated P22 Virus-like particles, that can be modified on their surface using sortase-mediated ligation. The potential of fine tuning the expression levels of both parts was tested via BBa_K3187011.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1499
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 98
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1499
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1499
  • 1000
    COMPATIBLE WITH RFC[1000]