Difference between revisions of "Part:BBa K3165041"
 
(One intermediate revision by one other user not shown)
Line 3: Line 3:
 
<partinfo>BBa_K3165041 short</partinfo>
 
<partinfo>BBa_K3165041 short</partinfo>
  
−
This part consists of the mini-CcaS protein complex with ECFP under the pCpcG2 promoter.
+
This part consists of the mini-CcaS protein complex <html><a  href="https://parts.igem.org/Part:BBa_K3165037">(BBa_K3165037)</a></html> with ECFP under the PcpcG2 promoter <html><a  href="https://parts.igem.org/Part:BBa_K3165040">(BBa_K3165040)</a></html>.
  
  
Line 12: Line 12:
 
===Usage and Biology===
 
===Usage and Biology===
  
−
The mini-CcaS protein consisting of a GAF domain, two PAS domains and a histidine kinase domain forms the light-sensitive part of the CcaS-CcaR system. ECFP placed downstream of the pCpcG2 is transcribed upon activation of the promoter by the response regulator CcaR mediated by the histidine kinase domain of CcaS. This part when used in accompaniment with the CcaR and Ho1-PcyA system will result in the translation of the complete photo-sensitive system.
+
The mini-CcaS protein consisting of a GAF domain, two PAS domains, and a histidine kinase domain forms the light-sensitive part of the CcaS-CcaR system. ECFP placed downstream of the PcpcG2 is transcribed upon activation of the promoter by the response regulator CcaR mediated by the histidine kinase domain of CcaS. This part when used in accompaniment with the CcaR and Ho1-PcyA system will result in the translation of the complete photo-sensitive system.
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  

Latest revision as of 18:46, 21 October 2019


mini-CcaS + ECFP

This part consists of the mini-CcaS protein complex (BBa_K3165037) with ECFP under the PcpcG2 promoter (BBa_K3165040).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Usage and Biology

The mini-CcaS protein consisting of a GAF domain, two PAS domains, and a histidine kinase domain forms the light-sensitive part of the CcaS-CcaR system. ECFP placed downstream of the PcpcG2 is transcribed upon activation of the promoter by the response regulator CcaR mediated by the histidine kinase domain of CcaS. This part when used in accompaniment with the CcaR and Ho1-PcyA system will result in the translation of the complete photo-sensitive system.