Generator

Part:BBa_K3165040

Designed by: Mohit Das   Group: iGEM19_IISc-Bangalore   (2019-10-19)


ECFP under PcpcG2 expression

This part places the fluorescent protein - ECFP (BBa_K3165006) under the inducible PcpcG2 promoter (BBa_K2012015).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Usage and Biology

This part encodes for the engineered cyan fluorescent protein (ECFP) under an inducible promoter PcpcG2. The promoter is activated by the phosphorylated form of CcaR (a response regulator) and deactivated by dephosphorylated CcaR. the phosphorylation/dephosphorylation of CcaR depends on the light that is shone and perceived by the trans-membrane CcaS protein. Upon light stimulation, the production of ECFP is triggered leading to fluorescence by the bacteria. By fluorescence measurements, the functioning of the light-sensitive system can be quantified. This part is used in association with the CcaS-CcaR system as a light-induced system to quantify the protein expression.


[edit]
Categories
Parameters
None