Difference between revisions of "Part:BBa K3015004"
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The iGEM Team BOKU-Vienna improved the Part [[Part:BBa_K784005|BBa_K784005]] by designing a Theophylline Riboswitch that works in a transcriptional way, instead of a translational way. | The iGEM Team BOKU-Vienna improved the Part [[Part:BBa_K784005|BBa_K784005]] by designing a Theophylline Riboswitch that works in a transcriptional way, instead of a translational way. | ||
| − | The coding sequence does not have to be scarless fused to the Riboswitch, which makes cloning easier. | + | The coding sequence does not have to be scarless fused to the Riboswitch, which makes cloning easier. <br> |
| − | To test the probabilities of the Riboswitch we conducted measurements by fusing the Ribswitch upstream to a Promotor and downstream to an RBS and GFP. You can find those measurements on the Registry-Page of the described Composite Part [[Part:BBa_K3015007|BBa_K3015007]] | + | Consists of an Aptamer-sequence, a spacer region, a terminator and a U-Stretch.<br> |
| + | To test the probabilities of the Riboswitch we conducted measurements by fusing the Ribswitch upstream to a Promotor and downstream to an RBS and GFP. You can find those measurements on the Registry-Page of the described Composite Part [[Part:BBa_K3015007|BBa_K3015007]]<br> | ||
| + | [[File:T--BOKU-Vienna--K3015001.png|600px]]<br> | ||
| + | Figure 1: This is the secondary structure of the Theophylline aptamer with intrinsic termination<br> | ||
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Latest revision as of 00:20, 22 October 2019
Theophylline_Riboswitch (transcriptional)
The iGEM Team BOKU-Vienna improved the Part BBa_K784005 by designing a Theophylline Riboswitch that works in a transcriptional way, instead of a translational way.
The coding sequence does not have to be scarless fused to the Riboswitch, which makes cloning easier.
Consists of an Aptamer-sequence, a spacer region, a terminator and a U-Stretch.
To test the probabilities of the Riboswitch we conducted measurements by fusing the Ribswitch upstream to a Promotor and downstream to an RBS and GFP. You can find those measurements on the Registry-Page of the described Composite Part BBa_K3015007
Figure 1: This is the secondary structure of the Theophylline aptamer with intrinsic termination
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]