Difference between revisions of "Part:BBa K137076"
 
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Using the Caltech iGEM [http://2008.igem.org/Team:Caltech/Protocols/Coculture_Inhibition_Assay coculture inhibition assay], a coculture of K137076 in pSB1A2 in JI377 was able to inhibit growth of JI377 containing pSB1AK3 (vs. no growth inhibition by cells containing an empty plasmid).
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===Functional Parameters===
 
===Functional Parameters===
 
<partinfo>BBa_K137076 parameters</partinfo>
 
<partinfo>BBa_K137076 parameters</partinfo>
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==Functional Parameters: Austin_UTexas==
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<body>
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<h3><center>Burden Imposed by this Part:</center></h3>
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<center><img src = "https://static.igem.org/mediawiki/parts/4/43/T--Austin_Utexas--Low_significant_burden.png" style = "width:200px;height:120px"></center>
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<figcaption><center><b>Burden Value: 9.5 ± 4.4% </b></center></figcaption>
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<p> Burden is the percent reduction in the growth rate of <i>E. coli</i> cells transformed with a plasmid containing this BioBrick (± values are 95% confidence limits). This part exhibited a significant burden. Users should be aware that BioBricks with a burden of >20-30% may be susceptible to mutating to become less functional or nonfunctional as an evolutionary consequence of this fitness cost. This risk increases as they used for more bacterial cell divisions or in larger cultures. Users should be especially careful when combining multiple burdensome parts, as plasmids with a total burden of >40% are expected to mutate so quickly that they become unclonable. Refer to any one of the
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<a href="https://parts.igem.org/Part:BBa_K3174002">BBa_K3174002</a> - <a href="https://parts.igem.org/Part:BBa_K3174007">BBa_K3174007</a> pages for more information on the methods and other conclusions from a large-scale measurement project conducted by the <a href="https://2019.igem.org/Team:Austin_UTexas">2019 Austin_UTexas team.</a></p>
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<p>This functional parameter was added by the <a href="https://2020.igem.org/Team:Austin_UTexas/Contribution">2020 Austin_UTexas team.</a></p>
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Latest revision as of 00:27, 4 September 2020

AHL inducible production of hydrogen peroxide

This part produces pyruvate oxidase (K137069) in response to detecting acylhomoserine lactones.


Usage and Biology

Using the Caltech iGEM [http://2008.igem.org/Team:Caltech/Protocols/H2O2_Production_Assay H2O2 production assay], K137076 in pSB1A2 in JI377 produces 800uM of H2O2 at 1 hour after induction (vs. negligible production for JI377 with an empty plasmid).

K137076.gif

Using the Caltech iGEM [http://2008.igem.org/Team:Caltech/Protocols/Coculture_Inhibition_Assay coculture inhibition assay], a coculture of K137076 in pSB1A2 in JI377 was able to inhibit growth of JI377 containing pSB1AK3 (vs. no growth inhibition by cells containing an empty plasmid).

K137076.png


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2741
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1005


Functional Parameters


Functional Parameters: Austin_UTexas

Burden Imposed by this Part:

Burden Value: 9.5 ± 4.4%

Burden is the percent reduction in the growth rate of E. coli cells transformed with a plasmid containing this BioBrick (± values are 95% confidence limits). This part exhibited a significant burden. Users should be aware that BioBricks with a burden of >20-30% may be susceptible to mutating to become less functional or nonfunctional as an evolutionary consequence of this fitness cost. This risk increases as they used for more bacterial cell divisions or in larger cultures. Users should be especially careful when combining multiple burdensome parts, as plasmids with a total burden of >40% are expected to mutate so quickly that they become unclonable. Refer to any one of the BBa_K3174002 - BBa_K3174007 pages for more information on the methods and other conclusions from a large-scale measurement project conducted by the 2019 Austin_UTexas team.

This functional parameter was added by the 2020 Austin_UTexas team.