Difference between revisions of "Part:BBa K137018"
 
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===Usage and Biology===
 
===Usage and Biology===
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When measured using the Caltech iGEM [http://2008.igem.org/Team:Caltech/Protocols/Flow_cytometry flow cytometry protocol], the response curve is shown below. 100nM AHL caused very slow growth, leading to poor protein expression.
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When measured using the Caltech iGEM [http://2008.igem.org/Team:Caltech/Protocols/Flow_cytometry flow cytometry protocol], K137018 in pSB1A2 in DH10B produces the response curve shown below. 100nM AHL caused very slow growth, leading to poor protein expression.
  
 
[[Image:K137018.gif]]
 
[[Image:K137018.gif]]
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===Functional Parameters===
 
===Functional Parameters===
 
<partinfo>BBa_K137018 parameters</partinfo>
 
<partinfo>BBa_K137018 parameters</partinfo>
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==Functional Parameters: Austin_UTexas==
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<html>
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<body>
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<h3><center>Burden Imposed by this Part:</center></h3>
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<figure>
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<div class = "center">
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<center><img src = "https://static.igem.org/mediawiki/parts/4/43/T--Austin_Utexas--Low_significant_burden.png" style = "width:200px;height:120px"></center>
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</div>
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<figcaption><center><b>Burden Value: 16.8 ± 8.2% </b></center></figcaption>
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</figure>
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<p> Burden is the percent reduction in the growth rate of <i>E. coli</i> cells transformed with a plasmid containing this BioBrick (± values are 95% confidence limits). This part exhibited a significant burden. Users should be aware that BioBricks with a burden of >20-30% may be susceptible to mutating to become less functional or nonfunctional as an evolutionary consequence of this fitness cost. This risk increases as they used for more bacterial cell divisions or in larger cultures. Users should be especially careful when combining multiple burdensome parts, as plasmids with a total burden of >40% are expected to mutate so quickly that they become unclonable. Refer to any one of the
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<a href="https://parts.igem.org/Part:BBa_K3174002">BBa_K3174002</a> - <a href="https://parts.igem.org/Part:BBa_K3174007">BBa_K3174007</a> pages for more information on the methods and other conclusions from a large-scale measurement project conducted by the <a href="https://2019.igem.org/Team:Austin_UTexas">2019 Austin_UTexas team.</a></p>
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<p>This functional parameter was added by the <a href="https://2020.igem.org/Team:Austin_UTexas/Contribution">2020 Austin_UTexas team.</a></p>
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  </p>
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Latest revision as of 00:39, 4 September 2020

AHL receiver + GFP

This parts produces GFP in response to detecting an acylhomoserinelactone (AHL). It was made to check that the RBS behind the luxR reciever was functional.


Usage and Biology

When measured using the Caltech iGEM [http://2008.igem.org/Team:Caltech/Protocols/Flow_cytometry flow cytometry protocol], K137018 in pSB1A2 in DH10B produces the response curve shown below. 100nM AHL caused very slow growth, leading to poor protein expression.

K137018.gif

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1005
    Illegal BsaI.rc site found at 1732


Functional Parameters


Functional Parameters: Austin_UTexas

Burden Imposed by this Part:

Burden Value: 16.8 ± 8.2%

Burden is the percent reduction in the growth rate of E. coli cells transformed with a plasmid containing this BioBrick (± values are 95% confidence limits). This part exhibited a significant burden. Users should be aware that BioBricks with a burden of >20-30% may be susceptible to mutating to become less functional or nonfunctional as an evolutionary consequence of this fitness cost. This risk increases as they used for more bacterial cell divisions or in larger cultures. Users should be especially careful when combining multiple burdensome parts, as plasmids with a total burden of >40% are expected to mutate so quickly that they become unclonable. Refer to any one of the BBa_K3174002 - BBa_K3174007 pages for more information on the methods and other conclusions from a large-scale measurement project conducted by the 2019 Austin_UTexas team.

This functional parameter was added by the 2020 Austin_UTexas team.