Difference between revisions of "Part:BBa K143053"
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<partinfo>BBa_K143053 short</partinfo> | <partinfo>BBa_K143053 short</partinfo> | ||
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Constitutive promoter veg(<bbpart>BBa_K143012</bbpart>) coupled to the strong Ribosome Binding Site spoVG(<bbpart>BBa_K143021</bbpart>) from ''B. subtilis''. | Constitutive promoter veg(<bbpart>BBa_K143012</bbpart>) coupled to the strong Ribosome Binding Site spoVG(<bbpart>BBa_K143021</bbpart>) from ''B. subtilis''. | ||
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Pveg-spoVG can be used in the context of a '''Ribosomes per second''' (RiPS) output generator | Pveg-spoVG can be used in the context of a '''Ribosomes per second''' (RiPS) output generator | ||
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'''To get the highest level of translation from this Promoter-RBS combination it must be connected to a coding region preceded by a coding region prefix<cite>1</cite>. A standard prefix will increase the distance between the RBS and the start codon, reducing translational efficiency.''' | '''To get the highest level of translation from this Promoter-RBS combination it must be connected to a coding region preceded by a coding region prefix<cite>1</cite>. A standard prefix will increase the distance between the RBS and the start codon, reducing translational efficiency.''' | ||
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
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− | <span class='h3bb'>Sequence and Features</span> | + | <span class='h3bb'><big>'''Sequence and Features'''</big></span> |
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<partinfo>BBa_K143053 SequenceAndFeatures</partinfo> | <partinfo>BBa_K143053 SequenceAndFeatures</partinfo> | ||
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<partinfo>BBa_K143053 parameters</partinfo> | <partinfo>BBa_K143053 parameters</partinfo> | ||
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+ | <span class='h3bb'><big>'''Part Characterisation'''</big></span> | ||
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+ | Please see the testing constructs [https://parts.igem.org/Part:BBa_K143079 BBa_K143079] and [https://parts.igem.org/wiki/index.php?title=Part:BBa_K143082 BBa_K143082] for characterisation data. | ||
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+ | ===References=== | ||
+ | <biblio> | ||
+ | #1 https://parts.igem.org/Assembly:RBS-CDS_issues | ||
+ | </biblio> |
Latest revision as of 22:23, 29 October 2008
Promoter Pveg and RBS spoVG for B. subtilis
Constitutive promoter veg(BBa_K143012) coupled to the strong Ribosome Binding Site spoVG(BBa_K143021) from B. subtilis.
Pveg-spoVG can be used in the context of a Ribosomes per second (RiPS) output generator
To get the highest level of translation from this Promoter-RBS combination it must be connected to a coding region preceded by a coding region prefix1. A standard prefix will increase the distance between the RBS and the start codon, reducing translational efficiency.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Part Characterisation
Please see the testing constructs BBa_K143079 and BBa_K143082 for characterisation data.
References
<biblio>
</biblio>