Difference between revisions of "Part:BBa K3015004"

Latest revision as of 00:20, 22 October 2019

Theophylline_Riboswitch (transcriptional)

The iGEM Team BOKU-Vienna improved the Part BBa_K784005 by designing a Theophylline Riboswitch that works in a transcriptional way, instead of a translational way. The coding sequence does not have to be scarless fused to the Riboswitch, which makes cloning easier.
Consists of an Aptamer-sequence, a spacer region, a terminator and a U-Stretch.
To test the probabilities of the Riboswitch we conducted measurements by fusing the Ribswitch upstream to a Promotor and downstream to an RBS and GFP. You can find those measurements on the Registry-Page of the described Composite Part BBa_K3015007

Figure 1: This is the secondary structure of the Theophylline aptamer with intrinsic termination

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 4: / Line 4: @@
 The iGEM Team BOKU-Vienna improved the Part [[Part:BBa_K784005|BBa_K784005]] by designing a Theophylline Riboswitch that works in a transcriptional way, instead of a translational way.
-Hereby, the coding sequence does not have to be scarless fused to the Riboswitch, which makes cloning easier.
+The coding sequence does not have to be scarless fused to the Riboswitch, which makes cloning easier. <br>
+Consists of an Aptamer-sequence, a spacer region, a terminator and a U-Stretch.<br>
+To test the probabilities of the Riboswitch we conducted measurements by fusing the Ribswitch upstream to a Promotor and downstream to an RBS and GFP. You can find those measurements on the Registry-Page of the described Composite Part [[Part:BBa_K3015007|BBa_K3015007]]<br>
+[[File:T--BOKU-Vienna--K3015001.png|600px]]<br>
+Figure 1: This is the secondary structure of the Theophylline aptamer with intrinsic termination<br>
 <!-- Add more about the biology of this part here