Difference between revisions of "Part:BBa K3015004"

 
 
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<partinfo>BBa_K3015004 short</partinfo>
 
<partinfo>BBa_K3015004 short</partinfo>
  
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The iGEM Team BOKU-Vienna improved the Part [[Part:BBa_K784005||BBa_K784005]] by designing a Theophylline Riboswitch that works in a transcriptional way, instead of a translational way.
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The iGEM Team BOKU-Vienna improved the Part [[Part:BBa_K784005|BBa_K784005]] by designing a Theophylline Riboswitch that works in a transcriptional way, instead of a translational way.
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Hereby, the coding sequence does not have to be scarless fused to the Riboswitch, which makes cloning easier.
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The coding sequence does not have to be scarless fused to the Riboswitch, which makes cloning easier. <br>
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Consists of an Aptamer-sequence, a spacer region, a terminator and a U-Stretch.<br>
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To test the probabilities of the Riboswitch we conducted measurements by fusing the Ribswitch upstream to a Promotor and downstream to an RBS and GFP. You can find those measurements on the Registry-Page of the described Composite Part [[Part:BBa_K3015007|BBa_K3015007]]<br>
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[[File:T--BOKU-Vienna--K3015001.png|600px]]<br>
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Figure 1: This is the secondary structure of the Theophylline aptamer with intrinsic termination<br>
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 00:20, 22 October 2019


Theophylline_Riboswitch (transcriptional)

The iGEM Team BOKU-Vienna improved the Part BBa_K784005 by designing a Theophylline Riboswitch that works in a transcriptional way, instead of a translational way. The coding sequence does not have to be scarless fused to the Riboswitch, which makes cloning easier.
Consists of an Aptamer-sequence, a spacer region, a terminator and a U-Stretch.
To test the probabilities of the Riboswitch we conducted measurements by fusing the Ribswitch upstream to a Promotor and downstream to an RBS and GFP. You can find those measurements on the Registry-Page of the described Composite Part BBa_K3015007
T--BOKU-Vienna--K3015001.png
Figure 1: This is the secondary structure of the Theophylline aptamer with intrinsic termination

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]