Difference between revisions of "Part:BBa K3052002"

 
 
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<partinfo>BBa_K3052002 short</partinfo>
 
<partinfo>BBa_K3052002 short</partinfo>
  
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The linalool synthase is used to convert geranyl pyrophosphate(GPP) to linalool, which has aroma of lavendar.
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The linalool synthase is used to convert geranyl pyrophosphate(GPP) to linalool, which has aroma of lavendar. This is a codon-optimized version based on E.coli.  
  
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K3052002 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3052002 SequenceAndFeatures</partinfo>
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<p><br />We have reached a cooperation with XJTU-China to test whether their LGF bacteria can work normally in 2019.
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<br />The LGF strain contains two plasmids, pGPP and pSB1K3-LGF. GPP can be synthesized by plasmid pGPP, while linalool can be synthesized by plasmid pSB1K3-LGF using GPP as substrate.
  
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<br />We qualitatively and quantitatively detected linalool in samples by gas chromatography.
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Test results are as follows:
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<br />(1) qualitative detection
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Chromatogram of linalool standard solution
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<br /><img src="https://parts.igem.org/File:T--NWU-CHINA-B--sepubiao.jpg" />
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<br />The retention time of linalool in the standard solution was 8.455min, and the peak area was 788,307.
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<br />Chromatogram of linalool sample
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<br /><img src="https://parts.igem.org/File:T--NWU-CHINA-B--sepuyangpin.jpg" />
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<br />The retention time of linalool in the sample was 8.431min, and the peak area was 632439. The sample contains a peak with a retention time close to that in the standard solution, which can be inferred as the product astragaloside.
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<br />(2) Quantitative detection
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<br />The quantitative measurement result of linalool is 377.66mg/ml.</p>
  
 
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Latest revision as of 03:11, 28 October 2020


(S)-linalool synthase: LGF

The linalool synthase is used to convert geranyl pyrophosphate(GPP) to linalool, which has aroma of lavendar. This is a codon-optimized version based on E.coli.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 298
    Illegal PstI site found at 817
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 298
    Illegal PstI site found at 817
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 129
    Illegal BglII site found at 1091
    Illegal BglII site found at 1139
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 298
    Illegal PstI site found at 817
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 298
    Illegal PstI site found at 817
    Illegal NgoMIV site found at 450
  • 1000
    COMPATIBLE WITH RFC[1000]


We have reached a cooperation with XJTU-China to test whether their LGF bacteria can work normally in 2019.
The LGF strain contains two plasmids, pGPP and pSB1K3-LGF. GPP can be synthesized by plasmid pGPP, while linalool can be synthesized by plasmid pSB1K3-LGF using GPP as substrate.
We qualitatively and quantitatively detected linalool in samples by gas chromatography. Test results are as follows:
(1) qualitative detection Chromatogram of linalool standard solution
<img src="https://parts.igem.org/File:T--NWU-CHINA-B--sepubiao.jpg" />
The retention time of linalool in the standard solution was 8.455min, and the peak area was 788,307.
Chromatogram of linalool sample
<img src="https://parts.igem.org/File:T--NWU-CHINA-B--sepuyangpin.jpg" />
The retention time of linalool in the sample was 8.431min, and the peak area was 632439. The sample contains a peak with a retention time close to that in the standard solution, which can be inferred as the product astragaloside.
(2) Quantitative detection
The quantitative measurement result of linalool is 377.66mg/ml.