Difference between revisions of "Part:BBa K145150"

Latest revision as of 11:15, 22 July 2009

Hybrid promoter: HSL-LuxR activated, P22 C2 repressed

Hybrid promoter consisting of the Lux box upstream the -35 box and binding sites for P22 C2; O_R2 and O_R1 located between the -35 and -10 boxes and downstream of them respectively. Transcription from this promoter can be activated by a LuxR-HSL complex and repressed by p22 C2. Background transcription from this pomoter (no activation and no repression) is very low.

Comment

Sequence read does not match part. Part was made with two primers and Klenow end-filling. These primers are available on [http://2008.igem.org/Team:KULeuven request].

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K145150 short</partinfo>
-Hybrid promoter consisting of the Lux box which overlaps partly with -35 box. Binding sites for P22 C2 O<sub>R2</sub> and O<sub>R1</sub> are located between the -35 and -10 boxes and downstream of them respectively.
+Hybrid promoter consisting of the Lux box upstream the -35 box and binding sites for P22 C2; O<sub>R2</sub> and O<sub>R1</sub> located between the -35 and -10 boxes and downstream of them respectively. Transcription from this promoter can be activated by a LuxR-HSL complex and repressed by p22 C2. Background transcription from this pomoter (no activation and no repression) is very low.
+Comment
+Sequence read does not match part. Part was made with two primers and Klenow end-filling. These [https://parts.igem.org/Part:BBa_K145150:Design primers] are available on [http://2008.igem.org/Team:KULeuven request].
 <!-- Add more about the biology of this part here