Part:BBa_K145150:Design

Hybrid promoter: HSL-LuxR activated, P22 C2 repressed

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

-35 and -10 boxes have been modified to lower the basal transcription rate. This part was constructing with an end-filling PCR reaction (with Klenow polymerase) of the following primers:

primer 1: CATCATGAAT TCGCGGCCGC TTCTAGACCT GTAGGATCGT ACAGGTTTAC TAAAGATTCC TTTAG
primer 2: TACTAGTAGC GGCCGCTGCA GGAAATTAAA GAACACTTAA ATTATAAACT AAAGGAATCT TTAG

Source

Synthetic