Difference between revisions of "Part:BBa K2598027"
 
(One intermediate revision by one other user not shown)
Line 3: Line 3:
 
<partinfo>BBa_K2598027 short</partinfo>
 
<partinfo>BBa_K2598027 short</partinfo>
  
−
This is a bacterial flavin-containing monooxygenase(bFMO). It can catalyse tryptophan and render it blue. This part van be used as a reporter gene and, in our project, paint.
+
This is a bacterial flavin-containing monooxygenase(bFMO). It can catalyse tryptophan and render it blue. This part van be used as a reporter gene and, in our project, paint. It is part of the GRB system, see more information from [https://parts.igem.org/Part:BBa_J23106 RGB System]
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
Line 25: Line 25:
 
<b>Figure 2</b> shows colors produced by enzymes that can combine with substrate in the medium. We changed the output genes of our light control system to genes encoding three kinds of enzymes, including gusA（BBa_K330002）, lacZ（BBa_I732005） and bFMO（BBa_K2598027） that can combine with substrate in the medium to produce red, green and blue color respectively.  
 
<b>Figure 2</b> shows colors produced by enzymes that can combine with substrate in the medium. We changed the output genes of our light control system to genes encoding three kinds of enzymes, including gusA（BBa_K330002）, lacZ（BBa_I732005） and bFMO（BBa_K2598027） that can combine with substrate in the medium to produce red, green and blue color respectively.  
 
<div>[[File:T—UCAS-China—enzyme2018.png|700px|thumb|center|<b>Figure 2:</b> Colors produced by enzymes that can combine with substrate in the medium]]</div>
 
<div>[[File:T—UCAS-China—enzyme2018.png|700px|thumb|center|<b>Figure 2:</b> Colors produced by enzymes that can combine with substrate in the medium]]</div>
−
 
−
 
−
We explored the relationship between fluorescent intensity and illumination intensity, which affects the shade of the color.
 
−
 
−
<b>Figure 3</b> shows the red fluorescent intensity of E. coli under  light of 620-630nm wavelength with different illumination intensity. We found when illuminated under around 961lux light, we can get the most red fluorescence. We also explore the relationship between green and blue  fluorescent intensity of E. coli under  light of 515-530nm wavelength and 460-470nm wavelength respectively and illumination intensity. The results are similar, that is, moderate intensity of light is most favorable for E. coli to express fluorescence.
 
−
 
−
<div>[[File:T—UCAS-China—RRR2018.png|700px|thumb|center|<b>Figure 3:</b>The relationship between the red fluorescent intensity of E. coli under light of 620-630nm wavelength and illumination intensity]]</div>
 

Latest revision as of 03:48, 18 October 2018


bFMO(bacterial flavin-containing monooxygenase)

This is a bacterial flavin-containing monooxygenase(bFMO). It can catalyse tryptophan and render it blue. This part van be used as a reporter gene and, in our project, paint. It is part of the GRB system, see more information from RGB System

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 157
    Illegal AgeI site found at 180
    Illegal AgeI site found at 484
    Illegal AgeI site found at 814
  • 1000
    COMPATIBLE WITH RFC[1000]


Characterization

Figure 1 shows results of gel electrophoresis of various parts after PCR with primer VF2\VR. The distance between primer binding sites and both ends of the parts are approximately 150 bp, thus rendering the product about 300 bp longer. The picture is edited to show a more compact photo.


Figure 2 shows colors produced by enzymes that can combine with substrate in the medium. We changed the output genes of our light control system to genes encoding three kinds of enzymes, including gusA（BBa_K330002）, lacZ（BBa_I732005） and bFMO（BBa_K2598027） that can combine with substrate in the medium to produce red, green and blue color respectively.

Figure 2: Colors produced by enzymes that can combine with substrate in the medium