Difference between revisions of "Part:BBa K2718010"

Latest revision as of 23:22, 17 October 2018

RBS (strong) Hma s

HmaS is a mandelate synthase which catalyzes the reaction phenylpyruvate + O2 => (S)Mandelate + CO2

Activity test

Design notes

It's a part whith BBa_B0030 like RBS. And coding sequence comes from Amycolatopsis orientalis. And the sequence is optimize for E.coli thanks to IDT tool

This biobrick exists with IPTG inducible promotor BBa_K2718011

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 546
Illegal BamHI site found at 655
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 495

Sources

Pugh, S., McKenna, R., Halloum, I., and Nielsen, D. R. (2015). EngineeringEscherichia coli for renewable benzyl alcohol production. Met. Eng. Commun.2, 39–45. doi: 10.1016/j.meteno.2015.06.002

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2718010 short</partinfo>
-HmaS is a 4-hydroxymandelate synthase which catalise phenylpyruvate + O2  => (S)Mandelate + CO2
+HmaS is a [https://www.uniprot.org/uniprot/G4V4S7 mandelate synthase] which catalyzes the reaction phenylpyruvate + O2  => (S)Mandelate + CO2
 ===Activity test===
@@ Line 11: / Line 11: @@
 === Design notes===
-It's a composite part whith [https://parts.igem.org/Part:BBa_B0030 BBa_B0030] like RBS.
+It's a part whith [https://parts.igem.org/Part:BBa_B0030 BBa_B0030] like RBS.
-And coding sequence comes from ''Amycolatopsis orientalis''. And the sequence is optimize for E.coli thanks to
+And coding sequence comes from ''Amycolatopsis orientalis''. And the sequence is optimize for ''E.coli'' thanks to
 [https://eu.idtdna.com/CodonOpt IDT tool]
@@ Line 23: / Line 23: @@
 <span class='h3bb'>Sequence and Features</span>
 <partinfo>BBa_K2718010 SequenceAndFeatures</partinfo>
 ===Sources===