Difference between revisions of "Part:BBa K2598052"
 
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<partinfo>BBa_K2598052 short</partinfo>
 
<partinfo>BBa_K2598052 short</partinfo>
  
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This part contains lacZ, bFMO and gusA under the regulation of T7 RNAPsigma fragment.We use this part as output signal and our 'paint'
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This part contains lacZ, bFMO and gusA under the regulation of T7 RNAPsigma fragment.We use this part as output signal and our 'paint'.  It is part of the GRB system, see more information from [https://parts.igem.org/Part:BBa_J23106 RGB System]
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
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<div>[[File:T—UCAS-China—LIGHT AND LIGHT.png |700px|thumb|center|<b>Figure 1:</b>Relationship between the wavelength of light exposed on liquid medium and the intensity of BFP, GFP and RFP E. coli expressed from left figure to right figure respectively]]</div>
 
<div>[[File:T—UCAS-China—LIGHT AND LIGHT.png |700px|thumb|center|<b>Figure 1:</b>Relationship between the wavelength of light exposed on liquid medium and the intensity of BFP, GFP and RFP E. coli expressed from left figure to right figure respectively]]</div>
  
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===Characterization===
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<b>Figure 2</b> shows the relationship between fluorescence intensity and excitation wavelength. The x-axis is wavelength of 10h illumination. The solid medium gradually emerged and the y-axis is RGB figure of fluorescence in illuminated solid medium. This curve illustrates how our system responses to different excitation wavelength, which perfectly meets our expectation. So this figure proves that our system and our parts can work well.
 
<b>Figure 2</b> shows the relationship between fluorescence intensity and excitation wavelength. The x-axis is wavelength of 10h illumination. The solid medium gradually emerged and the y-axis is RGB figure of fluorescence in illuminated solid medium. This curve illustrates how our system responses to different excitation wavelength, which perfectly meets our expectation. So this figure proves that our system and our parts can work well.
 
<div>[[File:T—UCAS-China—abc222.png|700px|thumb|center|<b>Figure 2:</b>Relationship between fluorescence intensity and excitation wavelength]]</div>
 
<div>[[File:T—UCAS-China—abc222.png|700px|thumb|center|<b>Figure 2:</b>Relationship between fluorescence intensity and excitation wavelength]]</div>
  
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===Characterization===
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<b>Figure 3</b> shows colors we got from the solid medium exposed under light, in which E. coli producing fluorescent protein grows.  When E .coli producing fluorescent protein are exposed under uniform light of single wavelength, the solid medium gradually emerged corresponding colors. And using color picker, we get many pure colors with predominant continuity.
 
<b>Figure 3</b> shows colors we got from the solid medium exposed under light, in which E. coli producing fluorescent protein grows.  When E .coli producing fluorescent protein are exposed under uniform light of single wavelength, the solid medium gradually emerged corresponding colors. And using color picker, we get many pure colors with predominant continuity.
 
<div>[[File:T—UCAS-China—FP SOLIDE.png|700px|thumb|center|<b>Figure 3:</b> Colors we got from the solid medium, in which E. coli producing fluorescent protein grows, exposed under light]]</div>
 
<div>[[File:T—UCAS-China—FP SOLIDE.png|700px|thumb|center|<b>Figure 3:</b> Colors we got from the solid medium, in which E. coli producing fluorescent protein grows, exposed under light]]</div>
  
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===Characterization===
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We explored the relationship between fluorescent intensity and illumination intensity, which affects the shade of the color.
 
We explored the relationship between fluorescent intensity and illumination intensity, which affects the shade of the color.
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<b>Figure 4</b> shows the red fluorescent intensity of ecoli under  light of 620-630nm wavelength with different illumination intensity. We found when illuminated under around 400-600lux light, we can get the most red fluorescence. We also explored the relationship between green and blue  fluorescent intensity of ecoli under  light of 515-530nm wavelength and 460-470nm wavelength respectively and illumination intensity. The results were similar, that is, moderate intensity of light is most favorable for E. coli to express fluorescence.
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<div>[[File:T—UCAS-China—RR2018.png|700px|thumb|center|<b>Figure 4:</b>The relationship between the red fluorescent intensity of ecoli under light of 620-630nm wavelength and illumination intensity]]</div>
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<b>Figure 4</b> shows the red fluorescent intensity of E. coli under  light of 620-630nm wavelength with different illumination intensity. We found when illuminated under around 961lux light, we can get the most red fluorescence. We also explore the relationship between green and blue  fluorescent intensity of E. coli under  light of 515-530nm wavelength and 460-470nm wavelength respectively and illumination intensity. The results are similar, that is, moderate intensity of light is most favorable for E. coli to express fluorescence.
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<div>[[File:T—UCAS-China—RRR2018.png|700px|thumb|center|<b>Figure 4:</b>The relationship between the red fluorescent intensity of E. coli under light of 620-630nm wavelength and illumination intensity]]</div>
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<b>Figure 5</b> shows colors produced by enzymes that can combine with substrate in the medium. We changed the output genes of our light control system to genes encoding three kinds of enzymes, including gusA（BBa_K330002）, lacZ（BBa_I732005） and bFMO（BBa_K2598027） that can combine with substrate in the medium to produce red, green and blue color respectively.
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<div>[[File:T—UCAS-China—enzyme2018.png|700px|thumb|center|<b>Figure 5:</b> Colors produced by enzymes that can combine with substrate in the medium]]</div>

Latest revision as of 03:54, 18 October 2018


lacZ+bFMO+gusA

This part contains lacZ, bFMO and gusA under the regulation of T7 RNAPsigma fragment.We use this part as output signal and our 'paint'. It is part of the GRB system, see more information from RGB System

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 3112
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 6208
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 5
    Illegal AgeI site found at 3879
    Illegal AgeI site found at 4209
    Illegal AgeI site found at 4513
    Illegal AgeI site found at 4536
  • 1000
    COMPATIBLE WITH RFC[1000]


Characterization

Figure 1 shows the relationship between the wavelength of light exposed on liquid medium and the intensity of BFP, GFP and RFP E. coli expressed from left figure to right figure respectively. We got the data through flow cytometer and analyzed it to get the figure. The y-axis is the number of cells, and the x-axis is fluorescence intensity. And every color is E. coli that grows for 8 hours under the light of the corresponding wavelength. We can see E. coli has the highest blue fluorescence expression under blue light from the left graph. And We can also see E. coli has the highest green and red fluorescence expression under green light and right light from the middle and right graph respectively. So this figure proves that our system and our parts can work well.

Figure 1:Relationship between the wavelength of light exposed on liquid medium and the intensity of BFP, GFP and RFP E. coli expressed from left figure to right figure respectively


Figure 2 shows the relationship between fluorescence intensity and excitation wavelength. The x-axis is wavelength of 10h illumination. The solid medium gradually emerged and the y-axis is RGB figure of fluorescence in illuminated solid medium. This curve illustrates how our system responses to different excitation wavelength, which perfectly meets our expectation. So this figure proves that our system and our parts can work well.

Figure 2:Relationship between fluorescence intensity and excitation wavelength


Figure 3 shows colors we got from the solid medium exposed under light, in which E. coli producing fluorescent protein grows. When E .coli producing fluorescent protein are exposed under uniform light of single wavelength, the solid medium gradually emerged corresponding colors. And using color picker, we get many pure colors with predominant continuity.

Figure 3: Colors we got from the solid medium, in which E. coli producing fluorescent protein grows, exposed under light


We explored the relationship between fluorescent intensity and illumination intensity, which affects the shade of the color.

Figure 4 shows the red fluorescent intensity of E. coli under light of 620-630nm wavelength with different illumination intensity. We found when illuminated under around 961lux light, we can get the most red fluorescence. We also explore the relationship between green and blue fluorescent intensity of E. coli under light of 515-530nm wavelength and 460-470nm wavelength respectively and illumination intensity. The results are similar, that is, moderate intensity of light is most favorable for E. coli to express fluorescence.

Figure 4:The relationship between the red fluorescent intensity of E. coli under light of 620-630nm wavelength and illumination intensity


Figure 5 shows colors produced by enzymes that can combine with substrate in the medium. We changed the output genes of our light control system to genes encoding three kinds of enzymes, including gusA（BBa_K330002）, lacZ（BBa_I732005） and bFMO（BBa_K2598027） that can combine with substrate in the medium to produce red, green and blue color respectively.

Figure 5: Colors produced by enzymes that can combine with substrate in the medium