Difference between revisions of "Part:BBa K2610041"

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===Usage and Biology===
 
===Usage and Biology===
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We, iGEM Leiden 2018,  have combined this naturally occurring constitutive promoter from Escherichia coli with the blue chromoprotein AmilCP (BBa_K592009) in order for us to compare it to our novel stress promoter+chromoprotein constructs. GapA is known for its high constitutive expression, as is demonstrated in Figure 1.
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We, iGEM Leiden 2018,  have combined this naturally occurring constitutive promoter ([[https://parts.igem.org/Part:BBa_K2610040 BBa_K2610040]) from <i> Escherichia coli </i> with the blue chromoprotein AmilCP ([[https://parts.igem.org/Part:BBa_K592009 BBa_K592009]) in order for us to compare it to our novel stress promoter+chromoprotein constructs.  
  
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Moreover, GapA being a natural occurring promoter there are still possibilities for optimization through radiation. This way variations of this promoter can provide access to an even wider spectrum of colors.
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Contrary to our stress promoters, this promoter has a constitutive high basal expression level. In contrast to many synthetic promoters, this novel promoter provides an indication of high levels of natural expression. Therefore, this promoter can be used as a future positive control of reporter production - such as GFP - in a natural situation. Moreover, GapA being a natural occurring promoter means that there are still possibilities for optimization through radiation. This way variations of this promoter can provide access to an even wider spectrum of colors.
  
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[[File:T--Leiden--gapaamilcp.png|750px]]
 
  
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<span style="font-size:1em"><b>Figure 1.</b> Constitutively expressed AmilCP </span>
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[[File:T--Leiden--chromo.png|600px]]
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<span style="font-size:1em"><b>Figure 1.</b> Constitutively expressed pGapA-AmilCP compared to non-transformed DH5a and pGapA-spis-Pink (pink chromoprotein)</span>
  
 
===References===
 
===References===

Latest revision as of 01:55, 18 October 2018


pGapA-AmilCP

Promoter GapA is naturally occurring constitutive promoter in Escherichia coli.

Usage and Biology

We, iGEM Leiden 2018, have combined this naturally occurring constitutive promoter ([BBa_K2610040) from Escherichia coli with the blue chromoprotein AmilCP ([BBa_K592009) in order for us to compare it to our novel stress promoter+chromoprotein constructs.

Contrary to our stress promoters, this promoter has a constitutive high basal expression level. In contrast to many synthetic promoters, this novel promoter provides an indication of high levels of natural expression. Therefore, this promoter can be used as a future positive control of reporter production - such as GFP - in a natural situation. Moreover, GapA being a natural occurring promoter means that there are still possibilities for optimization through radiation. This way variations of this promoter can provide access to an even wider spectrum of colors.


T--Leiden--chromo.png

Figure 1. Constitutively expressed pGapA-AmilCP compared to non-transformed DH5a and pGapA-spis-Pink (pink chromoprotein)

References

Munjal, N., Jawed, K., Wajid, S., & Yazdani, S. S. (2015). A constitutive expression system for cellulase secretion in Escherichia coli and its use in bioethanol production. PloS one, 10(3), e0119917.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 140