Difference between revisions of "Part:BBa J45999"
 
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<partinfo>BBa_J45999 short</partinfo>
 
<partinfo>BBa_J45999 short</partinfo>
  
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''Escherichia coli'' strain [http://cgsc2.biology.yale.edu/Strain.php?ID=64826 YYC912].  Biosafety level 1 organism derived from ''Escherichia coli'' K12.   
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''Escherichia coli'' strain [http://cgsc2.biology.yale.edu/Strain.php?ID=64826 YYC912] (<partinfo>BBa_J45999</partinfo>) is a biosafety level 1 organism derived from ''E. coli'' K12.  ''E. coli'' strain YYC912 is an odor-free chassis because it produces no detectable indole.  Indole is responsible for the foul smell of ''E. coli''.
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BBa_J45999 has a mutation in the ''tnaA'' gene which encodes a tryptophanase which converts tryptophan to indole.  Indole is responsible for the foul stench of ''E. coli'' so ''tnaA'' mutants can have reduced indole levels and thus reduced foul odor.
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===Usage and Biology===
 
===Usage and Biology===
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This ''E. coli'' strain is chloramphenicol-resistant.
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* CGSC#: 7602
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* Strain Designation
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**Designation: YYC912
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**Source Person: Cronan & Chang
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**Choice: 1 
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* Sex(Hfr,F+,F-,or F'): F-
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* Mutations
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**DE(argF-lac)169 
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**LAM- 
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**poxB15::lacZ::CmR  '''(Note chloramphenicol resistance)'''
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**IN(rrnD-rrnE)1 
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**rph-1 
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**tnaA5 
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* No. of Muts Carried: 6
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* Special Uses: lac fusion-stat.phase promoter
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* Has_Partner_Isogenic_WRT: poxB15::lacZ::CmR
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* Isogenic_Partner_Is: ZK126
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* References <cite>Chang-1994</cite> 
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* ID#: 64826
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<biblio>
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#Chang-1994 pmid=8022274
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</biblio>
  
 
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Latest revision as of 20:06, 9 March 2008

E. coli strain YYC912; Odor-free chassis

Escherichia coli strain [http://cgsc2.biology.yale.edu/Strain.php?ID=64826 YYC912] (BBa_J45999) is a biosafety level 1 organism derived from E. coli K12. E. coli strain YYC912 is an odor-free chassis because it produces no detectable indole. Indole is responsible for the foul smell of E. coli.

Usage and Biology

  • CGSC#: 7602
  • Strain Designation
    • Designation: YYC912
    • Source Person: Cronan & Chang
    • Choice: 1
  • Sex(Hfr,F+,F-,or F'): F-
  • Mutations
    • DE(argF-lac)169
    • LAM-
    • poxB15::lacZ::CmR (Note chloramphenicol resistance)
    • IN(rrnD-rrnE)1
    • rph-1
    • tnaA5
  • No. of Muts Carried: 6
  • Special Uses: lac fusion-stat.phase promoter
  • Has_Partner_Isogenic_WRT: poxB15::lacZ::CmR
  • Isogenic_Partner_Is: ZK126
  • References Chang-1994
  • ID#: 64826

<biblio>

  1. Chang-1994 pmid=8022274

</biblio>

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]