Difference between revisions of "Part:BBa K2810002"
 
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The RTBV promotor is cloned into the golden gate version of pSB1C3 ([https://parts.igem.org/Part:BBa_P10500 P10500]) using BsmBI and conforms to the [http://2016.igem.org/Resources/Plant_Synthetic_Biology/PhytoBricks Phytobrick standard]. It is flanked by BsaI sites together 5' GAGG and 3' TACT cloning sequences for subsequent cloning into a level 1 plasmid. This allows addition of an 5’ enhancer sequence upstream of any required cloning sequence.
 
The RTBV promotor is cloned into the golden gate version of pSB1C3 ([https://parts.igem.org/Part:BBa_P10500 P10500]) using BsmBI and conforms to the [http://2016.igem.org/Resources/Plant_Synthetic_Biology/PhytoBricks Phytobrick standard]. It is flanked by BsaI sites together 5' GAGG and 3' TACT cloning sequences for subsequent cloning into a level 1 plasmid. This allows addition of an 5’ enhancer sequence upstream of any required cloning sequence.
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<span class='h3bb'>Usage and Biology</span>
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This promoter is supposed to cause gene expression only in phloem tissue, as previously documented [https://www.ncbi.nlm.nih.gov/pubmed/8220476 here.] Its original host is the Rice Tungro Bacilliform Virus.
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K2810002 SequenceAndFeatures</partinfo>
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===Functional Parameters===
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<partinfo>BBa_K2810002 parameters</partinfo>
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Latest revision as of 10:47, 10 October 2018


RTBV Vascular Specific Promotor

The Rice Tungro Bacilliform Virus (RTBV) promotor has a vascular expression pattern in plants. This promotor has been added to the registry to facilitate expression of genes into plant vascular tissues.

The RTBV promotor is cloned into the golden gate version of pSB1C3 (P10500) using BsmBI and conforms to the [http://2016.igem.org/Resources/Plant_Synthetic_Biology/PhytoBricks Phytobrick standard]. It is flanked by BsaI sites together 5' GAGG and 3' TACT cloning sequences for subsequent cloning into a level 1 plasmid. This allows addition of an 5’ enhancer sequence upstream of any required cloning sequence.


Usage and Biology

This promoter is supposed to cause gene expression only in phloem tissue, as previously documented here. Its original host is the Rice Tungro Bacilliform Virus.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 611
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 495
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]