Difference between revisions of "Part:BBa K2258003"
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This part is useful as constitutively expressed a reporter. | This part is useful as constitutively expressed a reporter. | ||
| − | [[Image:T--IISER-Pune-India-- | + | [[Image:T--IISER-Pune-India--ChromophorePellet.jpg|300px]] |
| + | [[Image:T--IISER-Pune-India--LiquidCulture.jpg|300px]] | ||
| − | '''iGEM17_IISER-Pune-India:''' Expression of chromoproteins. The images above show ''E coli'' constitutively expressing | + | '''iGEM17_IISER-Pune-India:''' Expression of chromoproteins. The images above show ''E coli'' constitutively expressing RFP <partinfo>BBa_E1010</partinfo> (red), tsPurple <partinfo>BBa_K1033906</partinfo> (purple) and amilCP <partinfo>BBa_K592009</partinfo> (blue). |
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'''iGEM17_IISER-Pune-India:''' Absorbance of ''E.coli'' culture expressing AmilCP. The graph above shows photometric analysis of liquid culture of ''E.coli'' constitutively expressing AmilCP <partinfo>BBa_K592009</partinfo> (blue) with an absorption peak at 590nm. | '''iGEM17_IISER-Pune-India:''' Absorbance of ''E.coli'' culture expressing AmilCP. The graph above shows photometric analysis of liquid culture of ''E.coli'' constitutively expressing AmilCP <partinfo>BBa_K592009</partinfo> (blue) with an absorption peak at 590nm. | ||
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| + | [[Image:T--IISER-Pune-India--AmilCPabsorbancePeaknormallized.jpg|300px]] | ||
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| + | '''iGEM17_IISER-Pune-India:''' Absorbance of ''E.coli'' culture expressing AmilCP. The graph above shows photometric analysis of liquid culture of ''E.coli'' constitutively expressing AmilCP <partinfo>BBa_K592009</partinfo> (blue) the absorbance at different optical densities are normalized with respect to the peak absorbance. | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
Latest revision as of 22:34, 1 November 2017
pCAT_AmilCP
It expresses AmilCP constitutively under pCAT promoter PCAT promoter (BBa_I14033) Composite Amil CP (BBa_K1357009)
Usage and Biology
This part is useful as constitutively expressed a reporter.
iGEM17_IISER-Pune-India: Expression of chromoproteins. The images above show E coli constitutively expressing RFP BBa_E1010 (red), tsPurple BBa_K1033906 (purple) and amilCP BBa_K592009 (blue).
Photometric Characterization
iGEM17_IISER-Pune-India: Absorbance of E.coli culture expressing AmilCP. The graph above shows photometric analysis of liquid culture of E.coli constitutively expressing AmilCP BBa_K592009 (blue) with an absorption peak at 590nm.
iGEM17_IISER-Pune-India: Absorbance of E.coli culture expressing AmilCP. The graph above shows photometric analysis of liquid culture of E.coli constitutively expressing AmilCP BBa_K592009 (blue) the absorbance at different optical densities are normalized with respect to the peak absorbance.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]