Difference between revisions of "Part:BBa K2258003"

Latest revision as of 22:34, 1 November 2017

pCAT_AmilCP

It expresses AmilCP constitutively under pCAT promoter PCAT promoter (BBa_I14033) Composite Amil CP (BBa_K1357009)

Usage and Biology

This part is useful as constitutively expressed a reporter.

iGEM17_IISER-Pune-India: Expression of chromoproteins. The images above show E coli constitutively expressing RFP BBa_E1010 (red), tsPurple BBa_K1033906 (purple) and amilCP BBa_K592009 (blue).

Photometric Characterization

iGEM17_IISER-Pune-India: Absorbance of E.coli culture expressing AmilCP. The graph above shows photometric analysis of liquid culture of E.coli constitutively expressing AmilCP BBa_K592009 (blue) with an absorption peak at 590nm.

iGEM17_IISER-Pune-India: Absorbance of E.coli culture expressing AmilCP. The graph above shows photometric analysis of liquid culture of E.coli constitutively expressing AmilCP BBa_K592009 (blue) the absorbance at different optical densities are normalized with respect to the peak absorbance.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 6: / Line 6: @@
 PCAT promoter (BBa_I14033) Composite Amil CP (BBa_K1357009)
-<!-- Add more about the biology of this part here
+<!-- Add more about the biology of this part here -->
 ===Usage and Biology===
-This part is useful as a reporter.
+This part is useful as constitutively expressed a reporter.
-[[Image:pCATChromophores.jpeg|300px]]
+[[Image:T--IISER-Pune-India--ChromophorePellet.jpg|300px]]
+[[Image:T--IISER-Pune-India--LiquidCulture.jpg|300px]]
-'''iGEM17_IISER-Pune-India:''' Expression of chromoproteins. The images above show ''E coli'' constitutively expressing amilCP <partinfo>BBa_K592009</partinfo> (blue), tsPurple <partinfo>BBa_K1033906</partinfo> (purple) and RFP <partinfo>BBa_E1010</partinfo> (red).
+'''iGEM17_IISER-Pune-India:''' Expression of chromoproteins. The images above show ''E coli'' constitutively expressing RFP <partinfo>BBa_E1010</partinfo> (red), tsPurple <partinfo>BBa_K1033906</partinfo> (purple) and amilCP <partinfo>BBa_K592009</partinfo> (blue).
+<!-- Characterization of this part here -->
+===Photometric Characterization===
+[[Image:T--IISER-Pune-India--AmilCPabsorbance.jpg|300px]]
+'''iGEM17_IISER-Pune-India:''' Absorbance of ''E.coli'' culture expressing AmilCP. The graph above shows photometric analysis of liquid culture of ''E.coli'' constitutively expressing AmilCP <partinfo>BBa_K592009</partinfo> (blue) with an absorption peak at 590nm.
+[[Image:T--IISER-Pune-India--AmilCPabsorbancePeaknormallized.jpg|300px]]
+'''iGEM17_IISER-Pune-India:''' Absorbance of ''E.coli'' culture expressing AmilCP. The graph above shows photometric analysis of liquid culture of ''E.coli'' constitutively expressing AmilCP <partinfo>BBa_K592009</partinfo> (blue) the absorbance at different optical densities are normalized with respect to the peak absorbance.