Difference between revisions of "Part:BBa K2258003"

 
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PCAT promoter (BBa_I14033) Composite Amil CP (BBa_K1357009)
 
PCAT promoter (BBa_I14033) Composite Amil CP (BBa_K1357009)
  
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===Usage and Biology===
 
===Usage and Biology===
This part is useful as a reporter.
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This part is useful as constitutively expressed a reporter.
  
[[Image:pCATChromophores.jpeg|300px]]
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[[Image:T--IISER-Pune-India--ChromophorePellet.jpg|300px]]
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[[Image:T--IISER-Pune-India--LiquidCulture.jpg|300px]]
  
'''iGEM17_IISER-Pune-India:''' Expression of chromoproteins. The images above show ''E coli'' constitutively expressing amilCP <partinfo>BBa_K592009</partinfo> (blue), tsPurple <partinfo>BBa_K1033906</partinfo> (purple) and RFP <partinfo>BBa_E1010</partinfo> (red).
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'''iGEM17_IISER-Pune-India:''' Expression of chromoproteins. The images above show ''E coli'' constitutively expressing RFP <partinfo>BBa_E1010</partinfo> (red), tsPurple <partinfo>BBa_K1033906</partinfo> (purple) and amilCP <partinfo>BBa_K592009</partinfo> (blue).
  
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<!-- Characterization of this part here -->
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===Photometric Characterization===
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[[Image:T--IISER-Pune-India--AmilCPabsorbance.jpg|300px]]
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'''iGEM17_IISER-Pune-India:''' Absorbance of ''E.coli'' culture expressing AmilCP. The graph above shows photometric analysis of liquid culture of ''E.coli'' constitutively expressing AmilCP <partinfo>BBa_K592009</partinfo> (blue) with an absorption peak at 590nm.
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[[Image:T--IISER-Pune-India--AmilCPabsorbancePeaknormallized.jpg|300px]]
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'''iGEM17_IISER-Pune-India:''' Absorbance of ''E.coli'' culture expressing AmilCP. The graph above shows photometric analysis of liquid culture of ''E.coli'' constitutively expressing AmilCP <partinfo>BBa_K592009</partinfo> (blue) the absorbance at different optical densities are normalized with respect to the peak absorbance.
  
  

Latest revision as of 22:34, 1 November 2017


pCAT_AmilCP

It expresses AmilCP constitutively under pCAT promoter PCAT promoter (BBa_I14033) Composite Amil CP (BBa_K1357009)

Usage and Biology

This part is useful as constitutively expressed a reporter.

T--IISER-Pune-India--ChromophorePellet.jpg T--IISER-Pune-India--LiquidCulture.jpg

iGEM17_IISER-Pune-India: Expression of chromoproteins. The images above show E coli constitutively expressing RFP BBa_E1010 (red), tsPurple BBa_K1033906 (purple) and amilCP BBa_K592009 (blue).


Photometric Characterization

T--IISER-Pune-India--AmilCPabsorbance.jpg

iGEM17_IISER-Pune-India: Absorbance of E.coli culture expressing AmilCP. The graph above shows photometric analysis of liquid culture of E.coli constitutively expressing AmilCP BBa_K592009 (blue) with an absorption peak at 590nm.


T--IISER-Pune-India--AmilCPabsorbancePeaknormallized.jpg

iGEM17_IISER-Pune-India: Absorbance of E.coli culture expressing AmilCP. The graph above shows photometric analysis of liquid culture of E.coli constitutively expressing AmilCP BBa_K592009 (blue) the absorbance at different optical densities are normalized with respect to the peak absorbance.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]