Difference between revisions of "Part:BBa K2382013"

Latest revision as of 22:35, 1 November 2017

EAAAK+ RFP+Histag

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 606
1000
COMPATIBLE WITH RFC[1000]

Usage and Biology

This is a fragment of BBa_K2382010. We designed a restriction site, BamHI, before EAAAK rigid linker, so future iGEM teams could take advantage of this part to fuse their proteins with RFP as indicator.

Characterization of the EAAAK+ RFP+Histag

Improvement

This is an improvement of previous BioBrick Part BBa_E1010. It encodes a RFP that has the same amino acids as BBa_E1010 and having a His-Tag at the end.
This part has codon optimized for E.coli. And we removed the barcode, which contains stop codon, from BBa_E1010. We hope this design could help other teams to utilize RFP in their fusion protein design and be easily purified.

References

BioBrick Part BBa_E1010

@@ Line 29: / Line 29: @@
 ==Characterization of the EAAAK+ RFP+Histag==
-===Improvemen===
+===Improvement===
-This is also an improvement of previous BioBrick Part <partinfo>BBa_E1010</partinfo>. It encodes an RFP that have
+This is an improvement of previous BioBrick Part <partinfo>BBa_E1010</partinfo>. It encodes a RFP that has
-the same amino acids as BBa_E1010 and having a His Tag at the end. Therefore, a fusion protein
+the same amino acids as BBa_E1010 and having a His-Tag at the end. <br>
-with this part may have red color and the ability to be purified easily.
+This part has codon optimized for E.coli. And we removed the barcode, which contains stop codon, from BBa_E1010. We hope this design could help other teams to utilize RFP in their fusion protein design and be easily purified.
 ===References===
   BioBrick Part <partinfo>BBa_E1010</partinfo>