Difference between revisions of "Part:BBa K2381000"

Latest revision as of 13:24, 1 November 2017

pMagFast

pMag, a light-induced dimerization protein derived from VVD protein family, mutates at I52R/M55R. Its corresponding protein, nMag also derived from the VVD protein, mutates at I52D/M55G. They can heterodimerize under blue light irritation (470 nm, 3 mW/cm²). Their light sensing molecule is FAD which is normal in nearly all organisms. This part is pMag-Fast, whose dissociation rate is more rapid than the origin pMag. It should be used together with nMag BBa_K2381001.It's function is proved by parts BBa_K2381017
If you want to use it, remember to fuse this part with the part you are interested in through a 12aa-linker, and remember to add FAD.

Reference

Kawano, F., Suzuki, H., Furuya, A., & Sato, M. (2015). Engineered pairs of distinct photoswitches for optogenetic control of cellular proteins. Nat Commun, 6, 6256. doi:10.1038/ncomms7256

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 87
1000
COMPATIBLE WITH RFC[1000]

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 __NOTOC__
 <partinfo>BBa_K2381000 short</partinfo>
 <html>
-pMag, a light-induced dimerization protein derived from VVD protein family, mutates at I52R/M55R. Its corresponding protein, nMag also derived from the VVD protein, mutates at I52D/M55G. They can heterodimerize under blue light irritation (470 nm, 3 mW/cm<sup>2</sup>). Their light sensing molecule is NAD which is normal in nearly all organisms.
+pMag, a light-induced dimerization protein derived from VVD protein family, mutates at I52R/M55R. Its corresponding protein, nMag also derived from the VVD protein, mutates at I52D/M55G. They can heterodimerize under blue light irritation (470 nm, 3 mW/cm<sup>2</sup>). Their light sensing molecule is FAD which is normal in nearly all organisms.
-This part is pMag-Fast, whose dissociation rate is more rapid than the origin pMag. It's function is proved by parts
+This part is pMag-Fast, whose dissociation rate is more rapid than the origin pMag. It should be used together with nMag
-  <a href="https://parts.igem.org/Part:BBa_K2381001">BBa_K2381017</a>
+<a href="https://parts.igem.org/Part:BBa_K2381001">BBa_K2381001</a>.It's function is proved by parts
-<br/>If you want to use it, remember to fuse this part with the part you are interested in through a 12aa-linker.
+  <a href="https://parts.igem.org/Part:BBa_K2381017">BBa_K2381017</a>
+<br/>If you want to use it, remember to fuse this part with the part you are interested in through a 12aa-linker, and remember to add FAD.