Difference between revisions of "Part:BBa K2380002"
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<partinfo>BBa_K2380002 short</partinfo> | <partinfo>BBa_K2380002 short</partinfo> | ||
− | The [https://parts.igem.org/Part:BBa_K2380000 Chitin Synthase (CHS) NodC] from <i>Rhizobium leguminosarum</i> is a N-acetylglucosaminyl transferase which catalyzes the formation of chitin pentamers by using UDP-acetylglucosamine as donor and N-acetylglucosamine as acceptor. The system includes a RBS (BBa_K2380024) and the expression of NodC is regulated by the AraC promoter system ( | + | The [https://parts.igem.org/Part:BBa_K2380000 Chitin Synthase (CHS) NodC] from <i>Rhizobium leguminosarum</i> is a N-acetylglucosaminyl transferase which catalyzes the formation of chitin pentamers by using UDP-acetylglucosamine as donor and N-acetylglucosamine as acceptor. The system includes a RBS [https://parts.igem.org/Part:BBa_K2380024 (BBa_K2380024)] and the expression of NodC is regulated by the AraC promoter system [https://parts.igem.org/Part:BBa_K808000 (BBa_K808000)]. For further characterizations please visit [https://parts.igem.org/Part:BBa_K2380000 BBa_K2380000] or our [http://2017.igem.org/Team:TU_Darmstadt/project/chitin_synthase wiki]. |
<h2>Plasmidcard</h2> | <h2>Plasmidcard</h2> |
Latest revision as of 22:53, 1 November 2017
Chitin synthase NodC with RBS and AraC promoter system.
The Chitin Synthase (CHS) NodC from Rhizobium leguminosarum is a N-acetylglucosaminyl transferase which catalyzes the formation of chitin pentamers by using UDP-acetylglucosamine as donor and N-acetylglucosamine as acceptor. The system includes a RBS (BBa_K2380024) and the expression of NodC is regulated by the AraC promoter system (BBa_K808000). For further characterizations please visit BBa_K2380000 or our [http://2017.igem.org/Team:TU_Darmstadt/project/chitin_synthase wiki].
Plasmidcard
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1144
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 979
Illegal AgeI site found at 1613 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 961