Difference between revisions of "Part:pSB3K1:Design"

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(Design Notes)
 
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__NOTOC__
 
__NOTOC__
<partinfo>BBa_I739204 short</partinfo>
+
<partinfo>pSB3K1 short</partinfo>
  
<partinfo>BBa_I739204 SequenceAndFeatures</partinfo>
+
<partinfo>pSB3K1 SequenceAndFeatures</partinfo>
  
  
 
===Design Notes===
 
===Design Notes===
 +
 +
*'''[[User:Rshetty|RS]] 23:12, 3 September 2008 (UTC)''': The default insert for this vector is [[Part:BBa_I739400]].  The physical location for this vector needs to be remapped to [[Part:BBa_I739400]].  pSB3K1 is the vector that contains [[Part:BBa_I739400]].
  
 
===Source===
 
===Source===

Latest revision as of 23:12, 3 September 2008

pACYC177 converted into BioBrick vector (formerly BBa_I739202)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3043
    Illegal NheI site found at 1305
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 3049
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3043
    Illegal BamHI site found at 3037
    Illegal XhoI site found at 1669
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 3043
    Illegal suffix found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 3043
    Plasmid lacks a suffix.
    Illegal XbaI site found at 3058
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal AgeI site found at 895
    Illegal AgeI site found at 1219
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 190


Design Notes

Source

Bioprocess Laboratory at ETH Zurich

References