Difference between revisions of "Part:BBa K2382013"

 
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===Usage and Biology===
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<partinfo>BBa_K2382013 parameters</partinfo>
 
<partinfo>BBa_K2382013 parameters</partinfo>
 
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===Usage and Biology===
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This is a fragment of <partinfo>BBa_K2382010</partinfo>. We designed a restriction site, BamHI, before EAAAK rigid
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linker, so future iGEM teams could take advantage of this part to fuse their proteins with RFP as
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indicator.
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<html>
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</p>
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</html>
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__TOC__
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==Characterization of the EAAAK+ RFP+Histag==
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===Improvement===
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This is an improvement of previous BioBrick Part <partinfo>BBa_E1010</partinfo>. It encodes a RFP that has
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the same amino acids as BBa_E1010 and having a His-Tag at the end. <br>
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This part has codon optimized for E.coli. And we removed the barcode, which contains stop codon, from BBa_E1010. We hope this design could help other teams to utilize RFP in their fusion protein design and be easily purified.
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===References===
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BioBrick Part <partinfo>BBa_E1010</partinfo>

Latest revision as of 22:35, 1 November 2017

EAAAK+ RFP+Histag


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 606
  • 1000
    COMPATIBLE WITH RFC[1000]


Usage and Biology

This is a fragment of BBa_K2382010. We designed a restriction site, BamHI, before EAAAK rigid linker, so future iGEM teams could take advantage of this part to fuse their proteins with RFP as indicator.


Characterization of the EAAAK+ RFP+Histag

Improvement

This is an improvement of previous BioBrick Part BBa_E1010. It encodes a RFP that has the same amino acids as BBa_E1010 and having a His-Tag at the end.
This part has codon optimized for E.coli. And we removed the barcode, which contains stop codon, from BBa_E1010. We hope this design could help other teams to utilize RFP in their fusion protein design and be easily purified.

References

BioBrick Part BBa_E1010