Difference between revisions of "Part:BBa I732920"
 
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[AHL]->PoPs->[ARL4A0604] converter. when AHL is present, it could initiate the downstream transcription at Plux, thus serve as a signal converter, converting chemical signal to PoPs(refer to description of Part:BBa_J09855), and then pass the signal to the next device in form of ARL4A0604 concentration.  
 
[AHL]->PoPs->[ARL4A0604] converter. when AHL is present, it could initiate the downstream transcription at Plux, thus serve as a signal converter, converting chemical signal to PoPs(refer to description of Part:BBa_J09855), and then pass the signal to the next device in form of ARL4A0604 concentration.  
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<!-- Add more about the biology of this part here
 
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===Usage and Biology===
 
===Usage and Biology===
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==Functional Parameters: Austin_UTexas==
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===Functional Parameters===
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<html>
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<body>
 
<partinfo>BBa_I732920 parameters</partinfo>
 
<partinfo>BBa_I732920 parameters</partinfo>
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<h3><center>Burden Imposed by this Part:</center></h3>
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<figure>
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<div class = "center">
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<center><img src = "https://static.igem.org/mediawiki/parts/f/fa/T--Austin_Utexas--no_burden_icon.png" style = "width:160px;height:120px"></center>
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</div>
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<figcaption><center><b>Burden Value: -0.5 ± 0.7% </b></center></figcaption>
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</figure>
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<p> Burden is the percent reduction in the growth rate of <i>E. coli</i> cells transformed with a plasmid containing this BioBrick (± values are 95% confidence limits). This BioBrick did not exhibit a burden that was significantly greater than zero (i.e., it appears to have little to no impact on growth). Therefore, users can depend on this part to remain stable for many bacterial cell divisions and in large culture volumes. Refer to any one of the
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<a href="https://parts.igem.org/Part:BBa_K3174002">BBa_K3174002</a> - <a href="https://parts.igem.org/Part:BBa_K3174007">BBa_K3174007</a> pages for more information on the methods, an explanation of the sources of burden,  and other conclusions from a large-scale measurement project conducted by the <a href="http://2019.igem.org/Team:Austin_UTexas">2019 Austin_UTexas team</a>.</p>
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<p>This functional parameter was added by the <a href="https://2020.igem.org/Team:Austin_UTexas/Contribution">2020 Austin_UTexas team.</a></p>
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</body>
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</html>

Latest revision as of 02:42, 25 August 2020

B0015 J09855 I732835

[AHL]->PoPs->[ARL4A0604] converter. when AHL is present, it could initiate the downstream transcription at Plux, thus serve as a signal converter, converting chemical signal to PoPs(refer to description of Part:BBa_J09855), and then pass the signal to the next device in form of ARL4A0604 concentration.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1124


Functional Parameters: Austin_UTexas

BBa_I732920 parameters

Burden Imposed by this Part:

Burden Value: -0.5 ± 0.7%

Burden is the percent reduction in the growth rate of E. coli cells transformed with a plasmid containing this BioBrick (± values are 95% confidence limits). This BioBrick did not exhibit a burden that was significantly greater than zero (i.e., it appears to have little to no impact on growth). Therefore, users can depend on this part to remain stable for many bacterial cell divisions and in large culture volumes. Refer to any one of the BBa_K3174002 - BBa_K3174007 pages for more information on the methods, an explanation of the sources of burden, and other conclusions from a large-scale measurement project conducted by the 2019 Austin_UTexas team.

This functional parameter was added by the 2020 Austin_UTexas team.