Difference between revisions of "Part:BBa K648013"
 
(One intermediate revision by one other user not shown)
Line 8: Line 8:
 
<hr>
 
<hr>
 
====Additional Tag====
 
====Additional Tag====
−
UCL iGEM 2017 used this BioBrick for its designs and noticed that this BioBrick actually contains the GFP protein reporter (E0040) fused to a FLAG epitope tag, followed by an enterokinase cleavage site. This is important if cell surface expression of this BioBrick is wished. Addition of [https://en.wikipedia.org/wiki/Enteropeptidase enterokinase] (also called enteropeptidase) to the solution allows easy testing of protein localisation.
+
UCL iGEM 2017 used this BioBrick for its designs and noticed that this BioBrick actually contains the GFP protein reporter (E0040) fused to a FLAG epitope tag, followed by an enterokinase cleavage site. This is important if cell surface expression of this BioBrick is wished. Addition of [https://en.wikipedia.org/wiki/Enteropeptidase enterokinase] (also called enteropeptidase) to the solution allows easy testing of protein localisation (inside or outside of the membrane).
  
 
<hr>
 
<hr>
Line 21: Line 21:
  
  
−
<!-- Uncomment this to enable Functional Parameter display
+
 
−
===Functional Parameters===
+
 
 +
==Functional Parameters: Austin_UTexas==
 +
<html>
 +
<body>
 
<partinfo>BBa_K648013 parameters</partinfo>
 
<partinfo>BBa_K648013 parameters</partinfo>
−
<!-- -->
+
<h3><center>Burden Imposed by this Part:</center></h3>
 +
<figure>
 +
<div class = "center">
 +
<center><img src = "https://static.igem.org/mediawiki/parts/f/fa/T--Austin_Utexas--no_burden_icon.png" style = "width:160px;height:120px"></center>
 +
</div>
 +
<figcaption><center><b>Burden Value: 1.3 ± 1.4% </b></center></figcaption>
 +
</figure>
 +
<p> Burden is the percent reduction in the growth rate of <i>E. coli</i> cells transformed with a plasmid containing this BioBrick (± values are 95% confidence limits). This BioBrick did not exhibit a burden that was significantly greater than zero (i.e., it appears to have little to no impact on growth). Therefore, users can depend on this part to remain stable for many bacterial cell divisions and in large culture volumes. Refer to any one of the
 +
<a href="https://parts.igem.org/Part:BBa_K3174002">BBa_K3174002</a> - <a href="https://parts.igem.org/Part:BBa_K3174007">BBa_K3174007</a> pages for more information on the methods, an explanation of the sources of burden,  and other conclusions from a large-scale measurement project conducted by the <a href="http://2019.igem.org/Team:Austin_UTexas">2019 Austin_UTexas team</a>.</p>
 +
<p>This functional parameter was added by the <a href="https://2020.igem.org/Team:Austin_UTexas/Contribution">2020 Austin_UTexas team.</a></p>
 +
</body>
 +
</html>

Latest revision as of 20:02, 27 August 2020


GFP with Standard 25 Prefix/Suffix

This is the standard GFP protein reporter (E0040) cloned with the prefix/suffix required for standard 25 assembly. It can easily be used to create fusion proteins through this method.


Additional Tag

UCL iGEM 2017 used this BioBrick for its designs and noticed that this BioBrick actually contains the GFP protein reporter (E0040) fused to a FLAG epitope tag, followed by an enterokinase cleavage site. This is important if cell surface expression of this BioBrick is wished. Addition of enterokinase (also called enteropeptidase) to the solution allows easy testing of protein localisation (inside or outside of the membrane).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 641



Functional Parameters: Austin_UTexas

BBa_K648013 parameters

Burden Imposed by this Part:

Burden Value: 1.3 ± 1.4%

Burden is the percent reduction in the growth rate of E. coli cells transformed with a plasmid containing this BioBrick (± values are 95% confidence limits). This BioBrick did not exhibit a burden that was significantly greater than zero (i.e., it appears to have little to no impact on growth). Therefore, users can depend on this part to remain stable for many bacterial cell divisions and in large culture volumes. Refer to any one of the BBa_K3174002 - BBa_K3174007 pages for more information on the methods, an explanation of the sources of burden, and other conclusions from a large-scale measurement project conducted by the 2019 Austin_UTexas team.

This functional parameter was added by the 2020 Austin_UTexas team.