Difference between revisions of "Part:BBa I742111"

 
 
(3 intermediate revisions by 2 users not shown)
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_I742111 short</partinfo>
 
<partinfo>BBa_I742111 short</partinfo>
  
−
Ribosome binding site attached to Limonene synthase gene.
+
Ribosome binding site attached to Limonene Synthase gene (LIMS1) from ''Citrus limon''. Limonene synthase enzyme produces (+)-limonene from farnesyl diphosphate (an intermediate in the mevalonate pathway).  (+) Limonene is a component of lemon scent.
 +
 
 +
For a diagram of limonene biosynthesis and the biosynthesis of other lemon flavour compounds see [http://2007.igem.org/Edinburgh/Yoghurt/Design#Lemon_Flavour_Production Edinburgh Yoghurt Wiki]
  
−
Limonene synthase enzyme produces (+)-limonene from farnesyl diphosphate (intermediate in mevlonate pathway).  (+) Limonene is a component of lemon scent.
 
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
Line 15: Line 15:
  
  
−
<!-- Uncomment this to enable Functional Parameter display
+
 
−
===Functional Parameters===
+
 
 +
==Functional Parameters: Austin_UTexas==
 +
<html>
 +
<body>
 
<partinfo>BBa_I742111 parameters</partinfo>
 
<partinfo>BBa_I742111 parameters</partinfo>
−
<!-- -->
+
<h3><center>Burden Imposed by this Part:</center></h3>
 +
<figure>
 +
<div class = "center">
 +
<center><img src = "https://static.igem.org/mediawiki/parts/f/fa/T--Austin_Utexas--no_burden_icon.png" style = "width:160px;height:120px"></center>
 +
</div>
 +
<figcaption><center><b>Burden Value: 22.7 ± 13.7% </b></center></figcaption>
 +
</figure>
 +
<p> Burden is the percent reduction in the growth rate of <i>E. coli</i> cells transformed with a plasmid containing this BioBrick (± values are 95% confidence limits). This BioBrick did not exhibit a burden that was significantly greater than zero (i.e., it appears to have little to no impact on growth). Therefore, users can depend on this part to remain stable for many bacterial cell divisions and in large culture volumes. Refer to any one of the
 +
<a href="https://parts.igem.org/Part:BBa_K3174002">BBa_K3174002</a> - <a href="https://parts.igem.org/Part:BBa_K3174007">BBa_K3174007</a> pages for more information on the methods, an explanation of the sources of burden,  and other conclusions from a large-scale measurement project conducted by the <a href="http://2019.igem.org/Team:Austin_UTexas">2019 Austin_UTexas team</a>.</p>
 +
<p>This functional parameter was added by the <a href="https://2020.igem.org/Team:Austin_UTexas/Contribution">2020 Austin_UTexas team.</a></p>
 +
</body>
 +
</html>

Latest revision as of 19:05, 3 September 2020

Limonene synthase gene with ribosome binding site

Ribosome binding site attached to Limonene Synthase gene (LIMS1) from Citrus limon. Limonene synthase enzyme produces (+)-limonene from farnesyl diphosphate (an intermediate in the mevalonate pathway). (+) Limonene is a component of lemon scent.

For a diagram of limonene biosynthesis and the biosynthesis of other lemon flavour compounds see [http://2007.igem.org/Edinburgh/Yoghurt/Design#Lemon_Flavour_Production Edinburgh Yoghurt Wiki]


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 116



Functional Parameters: Austin_UTexas

BBa_I742111 parameters

Burden Imposed by this Part:

Burden Value: 22.7 ± 13.7%

Burden is the percent reduction in the growth rate of E. coli cells transformed with a plasmid containing this BioBrick (± values are 95% confidence limits). This BioBrick did not exhibit a burden that was significantly greater than zero (i.e., it appears to have little to no impact on growth). Therefore, users can depend on this part to remain stable for many bacterial cell divisions and in large culture volumes. Refer to any one of the BBa_K3174002 - BBa_K3174007 pages for more information on the methods, an explanation of the sources of burden, and other conclusions from a large-scale measurement project conducted by the 2019 Austin_UTexas team.

This functional parameter was added by the 2020 Austin_UTexas team.