Difference between revisions of "Part:BBa K2380002"

 
 
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<partinfo>BBa_K2380002 short</partinfo>
 
<partinfo>BBa_K2380002 short</partinfo>
  
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The Chitin Synthase (CHS) NodC from Rhizobium leguminosarum is a N-acetylglucosaminyl transferase which catalyzes the formation of chitin pentamers by using UDP-acetylglucosamine as donor and N-acetylglucosamine as acceptor. The system includes a RBS (BBa_K2380024) and the expression of NodC is regulated by the AraC promoter system (BBa_808000).
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The [https://parts.igem.org/Part:BBa_K2380000 Chitin Synthase (CHS) NodC] from <i>Rhizobium leguminosarum</i> is a N-acetylglucosaminyl transferase which catalyzes the formation of chitin pentamers by using UDP-acetylglucosamine as donor and N-acetylglucosamine as acceptor. The system includes a RBS [https://parts.igem.org/Part:BBa_K2380024 (BBa_K2380024)] and the expression of NodC is regulated by the AraC promoter system [https://parts.igem.org/Part:BBa_K808000 (BBa_K808000)]. For further characterizations please visit [https://parts.igem.org/Part:BBa_K2380000 BBa_K2380000] or our [http://2017.igem.org/Team:TU_Darmstadt/project/chitin_synthase wiki].
  
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<h2>Plasmidcard</h2>
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===Usage and Biology===
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https://static.igem.org/mediawiki/parts/4/4b/T--TU_Darmstadt--nodCAraC-r.png
  
 
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Latest revision as of 22:53, 1 November 2017


Chitin synthase NodC with RBS and AraC promoter system.

The Chitin Synthase (CHS) NodC from Rhizobium leguminosarum is a N-acetylglucosaminyl transferase which catalyzes the formation of chitin pentamers by using UDP-acetylglucosamine as donor and N-acetylglucosamine as acceptor. The system includes a RBS (BBa_K2380024) and the expression of NodC is regulated by the AraC promoter system (BBa_K808000). For further characterizations please visit BBa_K2380000 or our [http://2017.igem.org/Team:TU_Darmstadt/project/chitin_synthase wiki].

Plasmidcard

T--TU_Darmstadt--nodCAraC-r.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
    Illegal AgeI site found at 1613
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961