Difference between revisions of "Part:BBa I739015"

 
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<partinfo>BBa_I739015 short</partinfo>
 
<partinfo>BBa_I739015 short</partinfo>
  
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===Part Structure===
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<p>The Biobrick encodes [https://parts.igem.org/wiki/index.php/Help:Tag LVA]-tagged P22 cII ([https://parts.igem.org/wiki/index.php/Part:BBa_C0053 BBa_C0053]) and untagged EYFP ([https://parts.igem.org/wiki/index.php/Part:BBa_E0030 BBa_E0030]) under control of the double promoter [https://parts.igem.org/wiki/index.php/Part:BBa_I739102 BBa_I739102]. The ribosome binding sites [https://parts.igem.org/wiki/index.php/Part:BBa_B0034 BBa_B0034] are located upstream of each coding region. The transcription of P22 cII and EYFP is terminated by the double terminator [https://parts.igem.org/wiki/index.php/Part:BBa_B0015 BBa_B0015].</p>
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===Mode of Action===
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<p>P22 cII and EYFP production can be repressed by cI and/or TetR. If the inducer [http://openwetware.org/wiki/ATc anhydrotetracycline (ATc)] is added, tetR action is inhibited and the promoter gets derepressed. However, there is no inducer available which neutralizes the action of cI. </p>
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===Purpose===
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<p>This Biobrick was designed for the [http://2007.igem.org/ETHZ ETHZ iGEM 2007 project] and belongs to the reporting part of the system. In the project description, this part is a composite of [https://parts.igem.org/wiki/index.php/Part:BBa_I739004 ''Part 4''] and [https://parts.igem.org/wiki/index.php/Part:BBa_E0430 ''Part 5new''].</p>
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===Testing===
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<p>Checked for uniqueness of restriction enzyme cleavage sites:<br>
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Eco: ok<br>
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Xba: ok<br>
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Spe: ok<br>
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Pst: ok<br>
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</p>
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 19:17, 24 October 2007

Double regulated polycistronic P22 cII +LVA / EYFP -LVA expression cassette

Part Structure

The Biobrick encodes LVA-tagged P22 cII (BBa_C0053) and untagged EYFP (BBa_E0030) under control of the double promoter BBa_I739102. The ribosome binding sites BBa_B0034 are located upstream of each coding region. The transcription of P22 cII and EYFP is terminated by the double terminator BBa_B0015.

Mode of Action

P22 cII and EYFP production can be repressed by cI and/or TetR. If the inducer [http://openwetware.org/wiki/ATc anhydrotetracycline (ATc)] is added, tetR action is inhibited and the promoter gets derepressed. However, there is no inducer available which neutralizes the action of cI.

Purpose

This Biobrick was designed for the [http://2007.igem.org/ETHZ ETHZ iGEM 2007 project] and belongs to the reporting part of the system. In the project description, this part is a composite of Part 4 and Part 5new.

Testing

Checked for uniqueness of restriction enzyme cleavage sites:
Eco: ok
Xba: ok
Spe: ok
Pst: ok

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 289