Difference between revisions of "Part:BBa K2404003"
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<partinfo>BBa_K2404003 short</partinfo> | <partinfo>BBa_K2404003 short</partinfo> | ||
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The [https://apps.araport.org/thalemine/report.do?id=1025303 PR2 promotor] is a regulatory sequence from <i>Arabidopsis thaliana</i> that has been shown to [http://onlinelibrary.wiley.com/doi/10.1046/j.1365-313X.1996.10061089.x/full respond to salicylic acid]. | The [https://apps.araport.org/thalemine/report.do?id=1025303 PR2 promotor] is a regulatory sequence from <i>Arabidopsis thaliana</i> that has been shown to [http://onlinelibrary.wiley.com/doi/10.1046/j.1365-313X.1996.10061089.x/full respond to salicylic acid]. | ||
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This part conforms to the [http://2016.igem.org/Resources/Plant_Synthetic_Biology/PhytoBricks Phytobrick] standard and contains the 5' GGAG and 3' AATG sequences for cloning into level 1 plasmids. | This part conforms to the [http://2016.igem.org/Resources/Plant_Synthetic_Biology/PhytoBricks Phytobrick] standard and contains the 5' GGAG and 3' AATG sequences for cloning into level 1 plasmids. | ||
− | This part is has been cloned into the | + | This part is has been cloned into the [https://parts.igem.org/Part:BBa_P10500 P10500] plasmid using BsmBI. |
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
− | <partinfo> | + | <partinfo>BBa_K2404003 SequenceAndFeatures</partinfo> |
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===Functional Parameters=== | ===Functional Parameters=== | ||
− | <partinfo> | + | <partinfo>BBa_K2404003 parameters</partinfo> |
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Latest revision as of 21:20, 30 October 2017
PR2 shortened - a salicylic acid-induced promoter
The PR2 promotor is a regulatory sequence from Arabidopsis thaliana that has been shown to [http://onlinelibrary.wiley.com/doi/10.1046/j.1365-313X.1996.10061089.x/full respond to salicylic acid].
This promotor is taken from sequence -642 > -145 upstream of the PR2 translational start point.
This part conforms to the [http://2016.igem.org/Resources/Plant_Synthetic_Biology/PhytoBricks Phytobrick] standard and contains the 5' GGAG and 3' AATG sequences for cloning into level 1 plasmids.
This part is has been cloned into the P10500 plasmid using BsmBI.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 238
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1
Illegal BsaI.rc site found at 514